SLMAP Antibody (SJ-09): sc-100957

SLMAP Antibody (SJ-09) is a mouse monoclonal IgG2a antibody that detects SLMAP (sarcolemmal membrane-associated protein) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SLMAP is a crucial 828 amino acid single-pass type IV membrane protein primarily localized to the sarcolemma in non-replicating cells, where SLMAP plays a significant role in muscle tissue dynamics. During cellular cleavage, SLMAP relocates to centrosomes in a microtubule-dependent manner, highlighting SLMAP′s importance in cellular organization and function. SLMAP facilitates protein-protein interactions and is essential for myoblast fusion, which is vital for muscle development and repair. Structurally, SLMAP exists as a homodimeric integral membrane protein featuring two leucine zippers that form a 77 amino acid coiled-coil structure, along with a forkhead-associated (FHA) domain. The coiled-coil structure is particularly important for regulating neurotransmitter release, N-type calcium channels, and membrane fusion, while the FHA domain is involved in nuclear signaling, underscoring SLMAP′s multifaceted roles in cellular processes. Additionally, SLMAP is produced in eight isoforms through alternative splicing, which may contribute to SLMAP′s diverse functional capabilities in different cellular contexts.

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SLMAP Antibody (SJ-09) References:

1. A novel isoform of sarcolemmal membrane-associated protein (SLMAP) is a component of the microtubule organizing centre.  |  Guzzo, RM., et al. 2004. J Cell Sci. 117: 2271-81. PMID: 15126628
2. Molecular properties of cardiac tail-anchored membrane protein SLMAP are consistent with structural role in arrangement of excitation-contraction coupling apparatus.  |  Guzzo, RM., et al. 2005. Am J Physiol Heart Circ Physiol. 288: H1810-9. PMID: 15591093
3. Hydrophobic profiles of the tail anchors in SLMAP dictate subcellular targeting.  |  Byers, JT., et al. 2009. BMC Cell Biol. 10: 48. PMID: 19538755
4. A fungal sarcolemmal membrane-associated protein (SLMAP) homolog plays a fundamental role in development and localizes to the nuclear envelope, endoplasmic reticulum, and mitochondria.  |  Nordzieke, S., et al. 2015. Eukaryot Cell. 14: 345-58. PMID: 25527523
5. SLMAP-3 is downregulated in human dilated ventricles and its overexpression promotes cardiomyocyte response to adrenergic stimuli by increasing intracellular calcium.  |  Nader, M., et al. 2019. Can J Physiol Pharmacol. 97: 623-630. PMID: 30856349
6. Targeting SLMAP-ALK-a novel gene fusion in lung adenocarcinoma.  |  Pagan, C., et al. 2019. Cold Spring Harb Mol Case Stud. 5: PMID: 31160357
7. The SLMAP/Striatin complex: An emerging regulator of normal and abnormal cardiac excitation-contraction coupling.  |  Nader, M. 2019. Eur J Pharmacol. 858: 172491. PMID: 31233748
8. Combinatorial targeting of Hippo-STRIPAK and PARP elicits synthetic lethality in gastrointestinal cancers.  |  An, L., et al. 2022. J Clin Invest. 132: PMID: 35290241
9. Multiancestry Genome-Wide Association Study of Aortic Stenosis Identifies Multiple Novel Loci in the Million Veteran Program.  |  Small, AM., et al. 2023. Circulation. 147: 942-955. PMID: 36802703
10. Glucagon-Like Peptide-1 Receptor Agonist Protects Against Diabetic Cardiomyopathy by Modulating microRNA-29b-3p/SLMAP.  |  Fang, P., et al. 2023. Drug Des Devel Ther. 17: 791-806. PMID: 36936522

The preferred SLMAP antibody is SLMAP Antibody (B-9): sc-393336.