SLC35E3 Double Nickase Plasmid (h): sc-412539-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC35E3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC35E3 Double Nickase Plasmid (h) and SLC35E3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC35E3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC35E3 Double Nickase Plasmid (h) | sc-412539-NIC | 20 µg | $410.00 | |||
SLC35E3 Double Nickase Plasmid (h2) | sc-412539-NIC-2 | 20 µg | $410.00 |
SLC35E3 encodes a predicted solute carrier family 35 nucleotide-sugar transporter–like protein that is thought to participate in nucleotide-sugar handling within the secretory pathway, supporting glycosylation-associated processes in the endoplasmic reticulum and Golgi network. By influencing the availability of activated sugar donors, SLC35E3 may contribute to protein and lipid glycan biosynthesis, which can affect membrane trafficking, cell–cell interactions, and receptor maturation. Altered glycosylation is broadly linked to immune regulation, developmental phenotypes, and tumor-associated changes in cell signaling, making SLC35E3 a useful target for probing glycan-dependent biology. Functional interrogation of SLC35E3 can help clarify how nucleotide-sugar transport capacity interfaces with Golgi homeostasis and cellular stress responses.
SLC35E3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC35E3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC35E3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC35E3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC35E3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.