SIRP-α Antibody (OX41): sc-53115
- SIRP-α Antibody (OX41) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against peritoneal macrophages of rat origin
- recommended for detection of SIRP-α of mouse and rat origin by WB, IP, IF, IHC(P) and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See SIRP-α/β (A-1): sc-17803 for SIRP-α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SIRP-α Antibody (OX41) for WB and IHC(P) applications. These reagents are now offered in bundles with SIRP-α Antibody (OX41) (see ordering information below).
SIRP-α Antibody (OX41) is a mouse monoclonal IgG2a antibody that detects SIRP-α in mouse and rat samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). SIRP-α, also known as SIRP-α1, SIRP-α2, or SIRP-α3, is a crucial transmembrane glycoprotein that plays a significant role in regulating immune responses. SIRP-α is primarily located on the surface of immune cells, including macrophages and dendritic cells, where SIRP-α interacts with CD47, a "don′t eat me" signal that inhibits phagocytosis. This interaction is vital for maintaining self-tolerance and preventing autoimmunity, as SIRP-α helps to modulate the activity of immune cells in response to various stimuli. The extracellular domain of SIRP-α contains three immunoglobulin-like structures, which are essential for binding interactions, while the cytoplasmic region features multiple tyrosine phosphorylation sites that facilitate downstream signaling pathways. By understanding the function and location of SIRP-α, researchers can better explore implications in immune regulation and potential therapeutic targets for diseases characterized by immune dysregulation. Anti-SIRP-α antibody (OX41) is an invaluable tool for studying these processes in both basic and applied research settings.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SIRP-α Antibody (OX41) References:
- Engineered SIRPα variants as immunotherapeutic adjuvants to anticancer antibodies. | Weiskopf, K., et al. 2013. Science. 341: 88-91. PMID: 23722425
- The interaction between signal regulatory protein alpha (SIRPα) and CD47: structure, function, and therapeutic target. | Barclay, AN. and Van den Berg, TK. 2014. Annu Rev Immunol. 32: 25-50. PMID: 24215318
- Disrupting the CD47-SIRPα anti-phagocytic axis by a humanized anti-CD47 antibody is an efficacious treatment for malignant pediatric brain tumors. | Gholamin, S., et al. 2017. Sci Transl Med. 9: PMID: 28298418
- Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. | Logtenberg, MEW., et al. 2019. Nat Med. 25: 612-619. PMID: 30833751
- Loss of microglial SIRPα promotes synaptic pruning in preclinical models of neurodegeneration. | Ding, X., et al. 2021. Nat Commun. 12: 2030. PMID: 33795678
- Intratumoral SIRPα-deficient macrophages activate tumor antigen-specific cytotoxic T cells under radiotherapy. | Bian, Z., et al. 2021. Nat Commun. 12: 3229. PMID: 34050181
- CD47-SIRPα axis blockade in NASH promotes necroptotic hepatocyte clearance by liver macrophages and decreases hepatic fibrosis. | Shi, H., et al. 2022. Sci Transl Med. 14: eabp8309. PMID: 36417485
- Reprogramming of TAMs via the STAT3/CD47-SIRPα axis promotes acquired resistance to EGFR-TKIs in lung cancer. | Lu, J., et al. 2023. Cancer Lett. 564: 216205. PMID: 37146936
- Hypoxia-induced ZEB1 promotes cervical cancer immune evasion by strengthening the CD47-SIRPα axis. | Chen, XJ., et al. 2024. Cell Commun Signal. 22: 15. PMID: 38183060
- Sirpα on tumor-associated myeloid cells restrains antitumor immunity in colorectal cancer independent of its interaction with CD47. | Huang, C., et al. 2024. Nat Cancer. 5: 500-516. PMID: 38200243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRP-α Antibody (OX41) | sc-53115 | 200 µg/ml | $322.00 | |||
SIRP-α Antibody (OX41): m-IgG Fc BP-HRP Bundle | sc-536893 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIRP-α Antibody (OX41): m-IgGκ BP-HRP Bundle | sc-534065 | 200 µg Ab; 40 µg BP | $361.00 | |||
SIRP-α Antibody (OX41): m-IgG2a BP-HRP Bundle | sc-547893 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIRP-α Antibody (OX41) FITC | sc-53115 FITC | 200 µg/ml | $336.00 | |||
SIRP-α Antibody (OX41) PE | sc-53115 PE | 200 µg/ml | $349.00 |