Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SH-PTP2 Double Nickase Plasmid (h): sc-400260-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SH-PTP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SH-PTP2 Double Nickase Plasmid (h) and SH-PTP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTPN11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SH-PTP2 Antibody (B-1): sc-7384
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SH-PTP2 Double Nickase Plasmid (h) | sc-400260-NIC | 20 µg | $410.00 | |||
SH-PTP2 Double Nickase Plasmid (h2) | sc-400260-NIC-2 | 20 µg | $410.00 |
PTPN11 encodes SH-PTP2 (SHP2), a cytosolic protein tyrosine phosphatase that relays signals from activated receptor tyrosine kinases and cytokine receptors to downstream RAS–MAPK/ERK and PI3K–AKT pathways. Through its SH2 domains and catalytic PTP domain, SHP2 modulates phosphorylation-dependent protein interactions that control proliferation, differentiation, migration, and survival. PTPN11 function is also linked to JAK/STAT signaling dynamics and feedback regulation within growth factor signaling networks. Dysregulated PTPN11 activity or mutation has been associated with developmental syndromes and diverse oncogenic signaling contexts, making it a widely studied node in pathway rewiring and signal transduction research.
SH-PTP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTPN11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTPN11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTPN11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTPN11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.