SCP2 CRISPR/Cas9 KO Plasmid (m): sc-422828
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCP2 CRISPR/Cas9 KO Plasmid (m) | sc-422828 | 20 µg | $397.00 |
Overview
Mouse Scp2 encodes sterol carrier protein 2 (SCP2), a small lipid-transfer protein that binds and shuttles cholesterol, bile acid intermediates, and long-chain fatty acyl-CoAs within cells, with prominent roles in peroxisomal and cytosolic lipid handling. SCP2 supports intracellular lipid trafficking and contributes to peroxisomal β-oxidation, ether lipid metabolism, and sterol homeostasis, intersecting with broader lipid metabolic networks that influence membrane composition and oxidative stress responses. Altered SCP2 function has been associated with disrupted cholesterol and fatty acid utilization and is frequently studied in the context of metabolic dysregulation and lipid-driven cellular stress phenotypes. Scp2 is therefore relevant for mechanistic research on peroxisome biology, lipid transport, and metabolic pathway remodeling in disease-relevant cell types.
SCP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCP2 protein expression.
This CRISPR knockout system enables efficient generation of Scp2-deficient cell models for investigation of SCP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scp2 exon(s) critical for SCP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCP2 CRISPR/Cas9 KO Plasmid (m) and SCP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCP2 HDR Plasmid (m) and SCP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scp2 homology arms to support homology-directed repair at defined Scp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.