SCGF CRISPR/Cas9 KO Plasmid (m): sc-422813
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCGF CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCGF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCGF CRISPR/Cas9 KO Plasmid (m) | sc-422813 | 20 µg | $397.00 |
Overview
Clec11a encodes stem cell growth factor (SCGF), a secreted cytokine-like protein that supports hematopoiesis by promoting survival and proliferation of early progenitors in the bone marrow microenvironment. SCGF contributes to regulation of stem and progenitor cell maintenance, influencing lineage output and recovery following hematopoietic stress. Clec11a activity is commonly studied in the context of stromal–hematopoietic signaling networks and growth factor–driven pathways that shape myeloid and erythroid development. Dysregulation of SCGF-associated signaling has been linked to altered progenitor function and is relevant to mechanistic studies of hematologic disorders and bone marrow niche dysfunction in mouse models.
SCGF CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Clec11a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Clec11a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Clec11a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCGF protein expression.
This CRISPR knockout system enables efficient generation of Clec11a-deficient cell models for investigation of SCGF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Clec11a exon(s) critical for SCGF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Clec11a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCGF CRISPR/Cas9 KO Plasmid (m) and SCGF CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Clec11a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCGF HDR Plasmid (m) and SCGF HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Clec11a homology arms to support homology-directed repair at defined Clec11a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.