Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCF Double Nickase Plasmid (m): sc-421644-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCF Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SCF Double Nickase Plasmid (m) and SCF Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Kitl. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCF Antibody (G-3): sc-13126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCF Double Nickase Plasmid (m) | sc-421644-NIC | 20 µg | $410.00 |
Mouse Kitl encodes stem cell factor (SCF), a membrane-bound and soluble cytokine that signals through the KIT receptor tyrosine kinase to regulate survival, proliferation, and migration of hematopoietic progenitors, melanocytes, germ cells, and mast cells. SCF–KIT engagement activates PI3K–AKT, RAS–MAPK, and JAK/STAT signaling, shaping stem/progenitor maintenance, niche interactions, and lineage commitment. In vivo and cellular models link altered Kitl/SCF activity to defects in hematopoiesis, pigmentation, fertility, and mast cell biology, and it is frequently studied in contexts where KIT pathway dysregulation contributes to oncogenic and inflammatory phenotypes.
SCF Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Kitl locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Kitl. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Kitl function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Kitl-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.