SAPS1 CRISPR/Cas9 KO Plasmid (h): sc-406315
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAPS1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SAPS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAPS1 CRISPR/Cas9 KO Plasmid (h) | sc-406315 | 20 µg | $397.00 |
Overview
PPP6R1 encodes SAPS1, a regulatory subunit of protein phosphatase 6 (PP6) that helps direct catalytic activity toward specific substrates and subcellular compartments. Through PP6 complex assembly, SAPS1 contributes to serine/threonine dephosphorylation programs that influence cell cycle progression, mitotic spindle organization, and DNA damage response signaling. PP6-linked phosphoregulation intersects with stress-activated pathways and checkpoint control, shaping genome stability and proliferative capacity. Dysregulated PP6 regulatory subunits have been implicated in altered phosphorylation homeostasis observed across multiple disease contexts, including cancer-associated signaling rewiring.
SAPS1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PPP6R1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PPP6R1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PPP6R1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SAPS1 protein expression.
This CRISPR knockout system enables efficient generation of PPP6R1-deficient cell models for investigation of SAPS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PPP6R1 exon(s) critical for SAPS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PPP6R1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SAPS1 CRISPR/Cas9 KO Plasmid (h) and SAPS1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PPP6R1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SAPS1 HDR Plasmid (h) and SAPS1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PPP6R1 homology arms to support homology-directed repair at defined PPP6R1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.