Sall4 Double Nickase Plasmid (h): sc-401033-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sall4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sall4 Double Nickase Plasmid (h) and Sall4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SALL4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sall4 Antibody (G-3): sc-166033
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sall4 Double Nickase Plasmid (h) | sc-401033-NIC | 20 µg | $410.00 | |||
Sall4 Double Nickase Plasmid (h2) | sc-401033-NIC-2 | 20 µg | $410.00 |
SALL4 encodes Sall4, a C2H2 zinc-finger transcription factor that helps maintain pluripotency and regulates early developmental gene expression programs, including networks controlling self-renewal, lineage commitment, and chromatin state. Sall4 coordinates transcriptional circuitry with factors such as OCT4 and NANOG and interfaces with epigenetic regulators to shape enhancer and promoter activity during embryogenesis. In somatic contexts, dysregulated SALL4 expression is linked to altered differentiation and proliferative signaling and has been reported in multiple malignancies, making it a useful node for studying oncogenic transcriptional rewiring. Genetic perturbation of SALL4 supports mechanistic investigations of stem cell biology, cell fate transitions, and transcription factor–driven gene regulatory networks.
Sall4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SALL4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SALL4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SALL4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SALL4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.