Safranin T is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin T has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin T is used to stain for chromosomes. In late Palaeozoic pteridosperm cuticles Safranin T has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. Safranin T has also been used to detect mucin and cartilage.
1. Rosenberg, L. 1971. J Bone Joint Surg Am. 53: 69-82. PMID: 4250366 2. Krings, M. 2000. Rev. Palaeobot. Palynol. 108: 143-150. PMID: 10704641 3. Penney, D.P., et al. 2002. Biotech Histochem. 77: 237-275. PMID: 12564600
Gram stain procedure
• Prepare and heat-fix bacterial smears.
• Flood the slides crystal violet for one minute.
• Pour off excess dye and wash gently in tap water and drain the slide against a paper towel.
• Expose the smears to Gram's iodine (sc-358803) for one minute by washing with iodine, then adding more iodine and leaving it on the smear until the minute is over.
• Wash with tap water and drain carefully. (Do not blot.)
• Wash with 95% alcohol for 30 seconds.
• Wash with tap water at the end of the 30 seconds to stop the decolorization. Drain.
• Counterstain with 25% Safranin T(sc-203758) for 30 seconds.
• Wash, drain, blot, and examine under oil.
Soluble in water (50 mg/ml at 20° C), and alcohol.
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Is this the same as Safranin-O? Can it be used to detect chondrogenic differentiation of Mesenchymal Stem Cells?
Asked by: Ramakrushna
Yes this is the same as Safranin-O. We haven’t tested this chemical for that application, but we can provide some references listed below:
Krings M. The use of biological stains in the analysis of late Paleozoic pteridosperm cuticles.Rev Palaeobot Palynol.2000108(3-4), 143-150.
Rosenberg L. Chemical basis for the histological use of safranin O in the study of articular cartilage.J Bone Joint Surg Am.197153(1), 69-82.
Answered by: Technical Support
Date published: 2019-01-26
is Safranin T(CAS 477-73-6) soluble in dicholorobenzene?
and if not, what solvent is used for it?
Asked by: bahar
Thank you for your question. Safranin T (CAS 477-73-6) sc-203758 is soluble in water (50 mg/ml at 20° C), and ethanol. Please contact our technical service directly for more information.
Rated 5 out of
Krings et alKrings et al. (PubMed ID 10704641) used the biological stain, safranin T, to increase contrast and enhance overall visualization of complex epidermal tissues. -SCBT Publication Review
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