Date published: 2025-9-5
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RyR Antibody (F-1) - Western Blotting - Image 305782
RyR 抗体 (F-1): sc-376507. 对 Sol8 全细胞裂解液 (A) 和小鼠脑组织提取物 (B) 中 RyR 的表达进行 Western 印迹分析.
RyR Antibody (F-1) - Western Blotting - Image 305782
RyR 抗体 (F-1): sc-376507. 对 Sol8 全细胞裂解液 (A) 和小鼠脑组织提取物 (B) 中 RyR 的表达进行 Western 印迹分析.
RyR Antibody (F-1) - Western Blotting - Image 156165
RyR 抗体 (F-1): sc-376507. 大鼠心脏组织提取物中 RyR 表达的 Western 印迹分析.
RyR 抗体 (F-1): sc-376507. 对 Sol8 全细胞裂解液 (A) 和小鼠脑组织提取物 (B) 中 RyR 的表达进行 Western 印迹分析.

RyR 抗体 (F-1): sc-376507

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寫評論提問

说明书
  • RyR 抗体 F-1 是小鼠单克隆 IgG1 κ，RyR抗体, 在11篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的RyR的N-terminus
  • RyR 抗体 (F-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 skeletal muscle, cardiac muscle and brain ryanodine receptors
  • 抗RyR抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RyR (F-1): sc-376507的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成RyR 抗体 (F-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 RyR 抗体包括 RyR-1

RyR抗体（F-1）是一种IgG1 κ小鼠单克隆RyR抗体（也称为Ryanodine受体3抗体、脑Ryanodine受体-钙释放通道抗体、脑型Ryanodine受体抗体、3型Ryanodine受体抗体或RYR-3抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的RyR蛋白。RyR抗体（F-1）既有非偶联形式的抗RyR抗体，也有多种偶联形式的抗RyR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。二氢吡啶受体（DHPR）是一种对于骨骼肌的兴奋-收缩偶联至关重要的表面膜蛋白。DHPR和肌质网Ryanodine受体（RyR）是细胞内连接处的两个关键组成部分，其中表面膜的去极化转化为内部储存的Ca2+的释放。DHPR的α1亚基包含一个细胞质环，该环被认为参与与RyR的相互作用。DHPR α1亚基的磷酸化也被认为在DHPR和RyR的功能性相互作用中发挥作用。DHPR α1的突变导致兴奋-收缩解偶联，导致肌肉发育不良，发育中的骨骼肌完全失活。不表达RyR的细胞也缺乏兴奋-收缩偶联，并且表现出Ca2+电流密度降低数倍。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RyR 抗体 (F-1) 参考文献:

  1. 研究神经-肌肉在兴奋-收缩耦合水平上异常相互作用的遗传模型：突变肌肉发育不良。  |  Pinçon-Raymond, M., et al. 1990. J Physiol (Paris). 84: 82-7. PMID: 2193149
  2. Ca2+和Mg2对RyR通道门控的调节。  |  Laver, DR. 2018. Biophys Rev. 10: 1087-1095. PMID: 29926426
  3. 他汀类药物对RyR和RyR相关疾病的影响。  |  Haseeb, M. and Thompson, PD. 2021. J Appl Physiol (1985). 131: 661-671. PMID: 34166122
  4. 心肌细胞启动器RyR簇在β刺激增强Ca2+释放后点燃其邻居。  |  Demydenko, K. and Roderick, HL. 2022. Acta Physiol (Oxf). 234: e13798. PMID: 35147280
  5. RyR介导的海马健康和疾病中的钙释放。  |  Hidalgo, C. and Paula-Lima, A. 2024. Trends Mol Med. 30: 25-36. PMID: 37957056
  6. 骨骼肌三肽融合肽上单克隆抗体与二氢吡啶受体α1亚基细胞质II-III环的结合位点。  |  Fan, H., et al. 1995. Biochemistry. 34: 14893-901. PMID: 7578101
  7. 二氢吡啶受体 II-III 环肽的磷酸化调节骨骼肌钙释放通道的功能。有证据表明 Ser687 的 beta-OH 基团发挥着重要作用。  |  Lu, X., et al. 1995. J Biol Chem. 270: 18459-64. PMID: 7629172
  8. 在基因缺陷骨骼肌细胞系中形成不含二氢吡啶受体α亚基的三联体。  |  Powell, JA., et al. 1996. J Cell Biol. 134: 375-87. PMID: 8707823
  9. 骨骼肌和心肌中参与兴奋-收缩耦合的连接点的形成。  |  Flucher, BE. and Franzini-Armstrong, C. 1996. Proc Natl Acad Sci U S A. 93: 8101-6. PMID: 8755610
  10. 心脏和骨骼雷诺丁受体的功能无质性。  |  Nakai, J., et al. 1997. Proc Natl Acad Sci U S A. 94: 1019-22. PMID: 9023375
  11. 二氢吡啶受体α1-亚基的羧基末端肽抑制钙（2+）释放通道。  |  Slavik, KJ., et al. 1997. Am J Physiol. 272: C1475-81. PMID: 9176137
  12. 横纹肌的瑞诺丁受体：一种能进行多种相互作用的复杂通道。  |  Franzini-Armstrong, C. and Protasi, F. 1997. Physiol Rev. 77: 699-729. PMID: 9234963

订购信息

产品名称产品编号规格价格数量收藏夹

RyR 抗体 (F-1)

sc-376507
200 µg/ml
$316.00

RyR 抗体 (F-1) AC

sc-376507 AC
500 µg/ml, 25% agarose
$416.00

RyR 抗体 (F-1) HRP

sc-376507 HRP
200 µg/ml
$316.00

RyR 抗体 (F-1) FITC

sc-376507 FITC
200 µg/ml
$330.00

RyR 抗体 (F-1) PE

sc-376507 PE
200 µg/ml
$343.00

RyR 抗体 (F-1) Alexa Fluor® 488

sc-376507 AF488
200 µg/ml
$357.00

RyR 抗体 (F-1) Alexa Fluor® 546

sc-376507 AF546
200 µg/ml
$357.00

RyR 抗体 (F-1) Alexa Fluor® 594

sc-376507 AF594
200 µg/ml
$357.00

RyR 抗体 (F-1) Alexa Fluor® 647

sc-376507 AF647
200 µg/ml
$357.00

RyR 抗体 (F-1) Alexa Fluor® 680

sc-376507 AF680
200 µg/ml
$357.00

RyR 抗体 (F-1) Alexa Fluor® 790

sc-376507 AF790
200 µg/ml
$357.00

Hi,I have a RyR2 mutation only have 98 amino acids, but I saw this antibody against 1-300 amino acids from N-terminal. I'm not sure whether this antibody works. Could you send me a sample to test? If it works, our lab could buy it later. Thank you!

Asked by: Shanshan
Thank you for contacting Santa Cruz Biotechnology. To receive information about our antibody sample program please contact Technical service. Please call 800-457-3801.
Answered by: BlakeJ
Date published: 2025-04-04

Can this antibody be used to detect RyR in canine samples?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2022-01-19
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Rated 4 out of 5 by from Stains HEK cell constitutively express human RYR2Stains RYR2 in an HEK cell line constitutively expresses human RYR2 (1:100 dilution, green puncta outside the nucleus).
Date published: 2019-10-18
Rated 5 out of 5 by from Excellent on mouse heart tissueStrong and clean signal on mouse heart samples after ON incubation of the 1:10000 dil. ab!
Date published: 2017-11-27
Rated 5 out of 5 by from Works good for WB100 ug of rat LV loaded onto biorad 4-15% gradient gels. Transfer onto 0.02 nm nitrocellulose. Antibody diluted to 1:500. Probed with 1:10 000 LiCor IRDye 800CW anti-mouse secondary antibody.
Date published: 2017-09-25
Rated 5 out of 5 by from good for wbI dilute the antibody at 1:1000 and incubate at 4℃ overnight.
Date published: 2017-09-18
Rated 5 out of 5 by from Great AntibodyWe tried it in Hek 293 cells transiently expressed with RyR2 and worked greatly.
Date published: 2017-07-27
Rated 5 out of 5 by from Greeat results!!!Great Western Blot data of RyR expression in rat heart tissue homogenate. 3-8% Tris-Acetate gels. Ab dilution 1:1000, 1% milk 0,1% Tween TBS. 4 hs incubation.
Date published: 2017-05-04
Rated 5 out of 5 by from Versatile detectionThis antibody works with mouse, rat, and human. It will provide positive signal in several applications such as WB, IP, and IF.
Date published: 2017-01-27
Rated 5 out of 5 by from Great Western Blot data of RyR expressionGreat Western Blot data of RyR expression in Sol8 whole cell lysate and mouse brain tissue extract . -SCBT QC
Date published: 2015-02-17
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RyR 抗体 (F-1) is rated 4.9 out of 5 by 9.
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