RIP3 Antibody (Rippy-3): sc-56228
- RIP3 Antibody (Rippy-3) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
- raised against full length RIP3 of human origin
- recommended for detection of RIP3 of mouse, rat and human origin by WB, IP and ELISA
- See RIP3 (B-2): sc-374639 for RIP3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RIP3 Antibody (Rippy-3). This work is in progress.
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RIP3 Antibody (Rippy-3) is a mouse monoclonal IgG1 antibody that detects RIP3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). RIP3 plays a crucial role in the regulation of programmed cell death, specifically in the process of necroptosis, which is a form of regulated necrosis that can occur when apoptosis is inhibited. RIP3 is primarily located in the cytoplasm, where RIP3 interacts with various signaling pathways to mediate cell death in response to specific stimuli, such as tumor necrosis factor (TNF) signaling. RIP3′s cytoplasmic localization enables rapid response to cellular stress and initiates necroptotic signaling cascades, thereby influencing cell fate decisions in both normal physiological processes and pathological conditions, such as inflammation and cancer. Additionally, RIP3 forms complexes with other proteins, including receptor-interacting protein 1 (RIP1) and mixed lineage kinase domain-like protein (MLKL), which are essential for the execution of necroptosis. Anti-RIP3 antibody (Rippy-3) serves as an invaluable tool for researchers studying cell death mechanisms and their implications in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RIP3 Antibody (Rippy-3) References:
- RIP3, an energy metabolism regulator that switches TNF-induced cell death from apoptosis to necrosis. | Zhang, DW., et al. 2009. Science. 325: 332-6. PMID: 19498109
- Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. | Cho, YS., et al. 2009. Cell. 137: 1112-23. PMID: 19524513
- Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. | Sun, L., et al. 2012. Cell. 148: 213-27. PMID: 22265413
- RIP3 induces apoptosis independent of pronecrotic kinase activity. | Mandal, P., et al. 2014. Mol Cell. 56: 481-95. PMID: 25459880
- CaMKII is a RIP3 substrate mediating ischemia- and oxidative stress-induced myocardial necroptosis. | Zhang, T., et al. 2016. Nat Med. 22: 175-82. PMID: 26726877
- RIP3 promotes colitis-associated colorectal cancer by controlling tumor cell proliferation and CXCL1-induced immune suppression. | Liu, ZY., et al. 2019. Theranostics. 9: 3659-3673. PMID: 31281505
- A specific RIP3+ subpopulation of microglia promotes retinopathy through a hypoxia-triggered necroptotic mechanism. | He, C., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33836603
- Mosaic composition of RIP1-RIP3 signalling hub and its role in regulating cell death. | Chen, X., et al. 2022. Nat Cell Biol. 24: 471-482. PMID: 35256774
- Activated Drp1 regulates p62-mediated autophagic flux and aggravates inflammation in cerebral ischemia-reperfusion via the ROS-RIP1/RIP3-exosome axis. | Zeng, X., et al. 2022. Mil Med Res. 9: 25. PMID: 35624495
- p55γ degrades RIP3 via MG53 to suppress ischaemia-induced myocardial necroptosis and mediates cardioprotection of preconditioning. | Li, Z., et al. 2023. Cardiovasc Res. 119: 2421-2440. PMID: 37527538
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIP3 Antibody (Rippy-3) | sc-56228 | 50 µg/0.5 ml | $322.00 |