rhophilin Antibody (F-7): sc-166302
- rhophilin Antibody (F-7) is a mouse monoclonal IgG2a κ rhophilin antibody provided at 200 µg/ml
- raised against amino acids 119-194 mapping near the N-terminus of rhophilin isoform 3 of human origin
- rhophilin Antibody (F-7) is recommended for detection of rhophilin of human origin by WB, IP, IF and ELISA
- Anti-rhophilin Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for rhophilin Antibody (F-7) for WB applications. This reagent is now offered in a bundle with rhophilin Antibody (F-7) (see ordering information below).
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rhophilin Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects rhophilin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). rhophilin Antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. rhophilin protein plays a crucial role in regulating actin-based cytoskeletal structures, which are essential for various cellular processes, including cell shape, motility, and division. Specifically, rhophilin interacts with Rho GTPases, which are pivotal in controlling cytoskeleton dynamics. This interaction is vital for proper functioning of cellular processes such as cytokinesis, where contractile ring apparatus is formed. rhophilin′s ability to potentially recruit cytoskeletal components suggests initiation of signaling cascades critical for cellular responses to external stimuli. Additionally, rhophilin shows significant sequence identity with other Rho-binding proteins, indicating importance in Rho signaling pathway. Understanding rhophilin′s function and interactions can provide insights into cellular mechanics and disease implications, making rhophilin (F-7) antibody a valuable research tool for studying these processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
rhophilin Antibody (F-7) References:
- Rhophilin-1 is a key regulator of the podocyte cytoskeleton and is essential for glomerular filtration. | Lal, MA., et al. 2015. J Am Soc Nephrol. 26: 647-62. PMID: 25071083
- Rhophilin-associated tail protein 1 promotes migration and metastasis in triple negative breast cancer via activation of RhoA. | Liu, Q., et al. 2020. FASEB J. 34: 9959-9971. PMID: 32427399
- Overexpression of Rhophilin Rho GTPase-binding protein 2 promotes hepatocellular carcinoma. | Yuan, B., et al. 2020. Oncol Lett. 20: 382. PMID: 33154780
- Overexpression of rhophilin 2 promotes pancreatic ductal adenocarcinoma. | Bo, W., et al. 2021. Oncol Lett. 21: 76. PMID: 33365087
- Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha (TOP2A). | Zhou, Y., et al. 2021. Bioengineered. 12: 12003-12022. PMID: 34787052
- Purification and characterization of a fatty acid-activated protein kinase (PKN) from rat testis. | Kitagawa, M., et al. 1995. Biochem J. 310 (Pt 2): 657-64. PMID: 7654208
- A novel partner for the GTP-bound forms of rho and rac. | Madaule, P., et al. 1995. FEBS Lett. 377: 243-8. PMID: 8543060
- Protein kinase N (PKN) and PKN-related protein rhophilin as targets of small GTPase Rho. | Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126
- Identification of a putative target for Rho as the serine-threonine kinase protein kinase N. | Amano, M., et al. 1996. Science. 271: 648-50. PMID: 8571127
- The role of the unique motifs in the amino-terminal region of PKN on its enzymatic activity. | Kitagawa, M., et al. 1996. Biochem Biophys Res Commun. 220: 963-8. PMID: 8607876
- PKN associates and phosphorylates the head-rod domain of neurofilament protein. | Mukai, H., et al. 1996. J Biol Chem. 271: 9816-22. PMID: 8621664
- Characterization of the interaction between RhoA and the amino-terminal region of PKN. | Shibata, H., et al. 1996. FEBS Lett. 385: 221-4. PMID: 8647255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rhophilin Antibody (F-7) | sc-166302 | 200 µg/ml | $316.00 | |||
rhophilin Antibody (F-7): m-IgG2a BP-HRP Bundle | sc-545926 | 200 µg Ab; 10 µg BP | $354.00 | |||
rhophilin Antibody (F-7) AC | sc-166302 AC | 500 µg/ml, 25% agarose | $416.00 | |||
rhophilin Antibody (F-7) HRP | sc-166302 HRP | 200 µg/ml | $316.00 | |||
rhophilin Antibody (F-7) FITC | sc-166302 FITC | 200 µg/ml | $330.00 | |||
rhophilin Antibody (F-7) PE | sc-166302 PE | 200 µg/ml | $343.00 | |||
rhophilin Antibody (F-7) Alexa Fluor® 488 | sc-166302 AF488 | 200 µg/ml | $357.00 | |||
rhophilin Antibody (F-7) Alexa Fluor® 546 | sc-166302 AF546 | 200 µg/ml | $357.00 | |||
rhophilin Antibody (F-7) Alexa Fluor® 594 | sc-166302 AF594 | 200 µg/ml | $357.00 | |||
rhophilin Antibody (F-7) Alexa Fluor® 647 | sc-166302 AF647 | 200 µg/ml | $357.00 | |||
rhophilin Antibody (F-7) Alexa Fluor® 680 | sc-166302 AF680 | 200 µg/ml | $357.00 | |||
rhophilin Antibody (F-7) Alexa Fluor® 790 | sc-166302 AF790 | 200 µg/ml | $357.00 |