RhoGEF p115 Antibody (E-4): sc-166301

RhoGEF p115 Antibody (E-4) is a mouse monoclonal IgG3 antibody that detects RhoGEF p115 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RhoGEF p115 plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor (GEF) specifically for Rho family GTPases, which are essential for regulating various cellular processes, including cytoskeletal dynamics and cell migration. RhoGEF p115′s ability to selectively activate Rho GTPases, while not affecting Rac or Cdc42, highlights its importance in maintaining the specificity of signaling pathways that govern cell shape and movement. This specificity is vital for processes such as wound healing and immune responses, where precise control of cell behavior is necessary. RhoGEF p115 is predominantly located in the cytoplasm, where RhoGEF p115 interacts with other signaling molecules to facilitate the exchange of GDP for GTP, thus toggling the Rho GTPases between their active and inactive states. The regulation of these GTPases by RhoGEF p115 underscores its significance in cellular communication and the overall orchestration of cellular functions.

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RhoGEF p115 Antibody (E-4) References:

1. Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF.  |  Zhang, H., et al. 1999. Curr Biol. 9: 1271-4. PMID: 10556093
2. Galpha 13 requires palmitoylation for plasma membrane localization, Rho-dependent signaling, and promotion of p115-RhoGEF membrane binding.  |  Bhattacharyya, R. and Wedegaertner, PB. 2000. J Biol Chem. 275: 14992-9. PMID: 10747909
3. Identification of potential mechanisms for regulation of p115 RhoGEF through analysis of endogenous and mutant forms of the exchange factor.  |  Wells, CD., et al. 2001. J Biol Chem. 276: 28897-905. PMID: 11384980
4. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor.  |  García-Mata, R. and Sztul, E. 2003. EMBO Rep. 4: 320-5. PMID: 12634853
5. Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor.  |  Kristelly, R., et al. 2004. J Biol Chem. 279: 47352-62. PMID: 15331592
6. A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin.  |  Dubash, AD., et al. 2007. J Cell Sci. 120: 3989-98. PMID: 17971419
7. Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF.  |  Chen, Z., et al. 2011. Protein Sci. 20: 107-17. PMID: 21064165
8. Mechanistic insights into specificity, activity, and regulatory elements of the regulator of G-protein signaling (RGS)-containing Rho-specific guanine nucleotide exchange factors (GEFs) p115, PDZ-RhoGEF (PRG), and leukemia-associated RhoGEF (LARG).  |  Jaiswal, M., et al. 2011. J Biol Chem. 286: 18202-12. PMID: 21454492
9. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation.  |  Singh, NK., et al. 2017. J Biol Chem. 292: 14080-14091. PMID: 28655771
10. The RHO Family GTPases: Mechanisms of Regulation and Signaling.  |  Mosaddeghzadeh, N. and Ahmadian, MR. 2021. Cells. 10: PMID: 34359999

The preferred RhoGEF p115 antibody is RhoGEF p115 Antibody (C-9): sc-74565.