Reverse Transcriptase, M-MuLV: sc-43500

Reverse Transcriptase, M-MuLV (Moloney Murine Leukemia Virus Reverse Transcriptase) is a crucial enzyme used in molecular biology for converting RNA into complementary DNA (cDNA), particularly useful in the study of RNA sequences and gene expression. Originating from the Moloney Murine Leukemia Virus, a retrovirus known for its robust reverse transcription mechanisms, M-MuLV Reverse Transcriptase possesses a unique attribute in that it lacks RNase H activity. This absence allows the enzyme to synthesize longer and more complete cDNA fragments by not degrading the RNA strand of the RNA-DNA hybrid during transcription. This capability is essential for applications requiring full-length cDNA for accurate gene transcription analysis, such as creating cDNA libraries, studying RNA viruses, and conducting transcriptome analyses through quantitative RT-PCR. Researchers rely on this enzyme to transcribe mRNA into DNA, facilitating further genetic analysis and manipulation in various studies, including gene expression profiling and molecular cloning. The enzyme′s high fidelity in synthesizing cDNA from RNA templates makes it a valuable tool in genetic research, where precise replication and transcription of genetic material are critical for understanding complex biological processes and mechanisms.