Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Raptor Double Nickase Plasmid (h): sc-400960-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Raptor Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Raptor Double Nickase Plasmid (h) and Raptor Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RPTOR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Raptor Antibody (A-2): sc-518004
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Raptor Double Nickase Plasmid (h) | sc-400960-NIC | 20 µg | $410.00 | |||
Raptor Double Nickase Plasmid (h2) | sc-400960-NIC-2 | 20 µg | $410.00 |
RPTOR encodes Raptor, a core scaffolding subunit of mTOR complex 1 (mTORC1) that recruits substrates and coordinates nutrient, growth factor, and energy signals to regulate protein synthesis, autophagy, ribosome biogenesis, and lipid metabolism. Through phosphorylation of downstream effectors such as S6K and 4E-BP1, Raptor-dependent mTORC1 activity shapes cell growth and proliferation programs and integrates lysosomal signaling with anabolic control. Dysregulated mTORC1/Raptor signaling has been implicated in cancers, metabolic disorders, and neurodevelopmental phenotypes, where altered translational control and autophagy contribute to disease-associated cellular states. As a central node in the PI3K–AKT–mTOR axis and amino acid sensing pathways, Raptor is widely studied in stress responses, mitochondrial and lysosomal homeostasis, and signaling cross-talk.
Raptor Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RPTOR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RPTOR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RPTOR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RPTOR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.