Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rad21 Double Nickase Plasmid (h): sc-402348-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rad21 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rad21 Double Nickase Plasmid (h) and Rad21 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAD21. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rad21 Antibody (B-2): sc-271601
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad21 Double Nickase Plasmid (h) | sc-402348-NIC | 20 µg | $410.00 | |||
Rad21 Double Nickase Plasmid (h2) | sc-402348-NIC-2 | 20 µg | $410.00 |
RAD21 encodes Rad21, a core component of the cohesin complex that forms a ring-like structure to tether sister chromatids and organize chromatin architecture. Rad21 supports accurate chromosome segregation, DNA replication dynamics, and DNA double-strand break repair through pathways that coordinate cell-cycle progression and genome maintenance. By shaping long-range chromatin interactions, RAD21 also contributes to transcriptional regulation at cohesin/CTCF-defined boundaries. Dysregulation or mutation of cohesin components, including RAD21, is associated with cohesinopathies and has been implicated in genomic instability phenotypes relevant to cancer biology.
Rad21 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAD21 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAD21. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAD21 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAD21-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.