RACK1 Antibody (B-3): sc-17754

RACK1 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that specifically targets amino acids 131-317 of RACK1, also known as Receptor for Activated C Kinase 1, and GNB2L1. RACK1 is a 317-amino-acid multifunctional scaffold protein resembling the G protein β subunit, playing a crucial role in various cellular processes such as cell growth, differentiation, and signal transduction. RACK1 functions as an anchoring protein for activated Protein Kinase C (PKC), facilitating PKC′s translocation and interaction with substrates, an essential process for regulating signaling pathways that control gene expression, hormone secretion, and membrane functions. By inhibiting Src tyrosine kinases and interacting with numerous signaling molecules, RACK1 modulates pathways important for cell adhesion, migration, and proliferation, making RACK1 significant in studies of cancer and other diseases. Anti-RACK1 antibody (B-3) reacts with RACK1 from mouse, rat, human, and additional species including equine, canine, bovine, porcine, and avian, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). RACK1′s role as a scaffold protein is vital for coordinating multiple signaling pathways, and RACK1′s conservation across diverse species underscores RACK1′s importance in fundamental cellular functions. Notably, deficits in RACK1 may be associated with impaired PKC activation in the aging brain, highlighting RACK1′s relevance in neurological studies. Therefore, RACK1 (B-3) antibody provides a valuable tool for advancing research in critical cellular mechanisms and exploring potential therapeutic targets, aiding in the understanding of diseases where RACK1 is implicated.

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RACK1 Antibody (B-3) References:

1. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
2. Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC.  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
3. Expression and characterization of protein kinase C-delta.  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
4. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
5. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
6. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
7. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
8. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RACK1 Antibody (B-3) has 92 citations in a variety of scientific publications.

Primary antibodies like Anti-RACK1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RACK1 Antibody (B-3) Specifications

Alternate Product Name: RACK1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 131-317

Epitope Target: RACK1

Epitope Species: human

Product Reactivity/Detection: RACK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 92 citations in various scientific publications

Available Conjugates/Forms for RACK1 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin