Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Purα CRISPR Activation Plasmid (h): sc-403760-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Purα CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Purα CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Purα CRISPR Activation Plasmid (h) and Purα CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the PURA transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Purα Antibody (80-L): sc-130397
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Purα CRISPR Activation Plasmid (h) | sc-403760-ACT | 20 µg | $397.00 |
PURA encodes Purα, a sequence-specific single-stranded DNA and RNA-binding protein that coordinates transcriptional regulation, DNA replication, and mRNA transport/translation. Purα participates in cell cycle control and genome maintenance and is enriched in neuronal contexts where it influences RNA granule dynamics and synaptic function. Dysregulation of PURA has been associated with neurodevelopmental phenotypes and altered neuronal excitability, and Purα-dependent RNA metabolism links this factor to broader pathways governing protein synthesis and stress responses. These properties make PURA a useful target for studying gene expression programs and RNA-protein interactions relevant to nervous system biology.
Purα CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous PURA expression without altering the underlying DNA sequence.
Purα CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the PURA locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the PURA transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Purα expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native PURA locus and enabling the study of Purα-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Purα pathway restoration in tumor cells with silenced or reduced PURA expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.