PRPF3 Antibody (42-N): sc-101130

PRPF3 Antibody (42-N) is a mouse monoclonal IgG3 kappa light chain antibody that detects PRPF3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). PRPF3 (42-N) antibody is available as the non-conjugated anti-PRPF3 monoclonal isotype antibody. PRPF3 protein, also known as PRP3 pre-mRNA processing factor 3 homolog, RP18, PRP3, Prp3p, HPRP3, or HPRP3P, plays a crucial role in pre-mRNA splicing as a component of the U4/U6.U5 tri-snRNP complex, which is essential for accurate processing of pre-mRNA into mature mRNA. PRPF3 is ubiquitously expressed, with significant levels found in retina, blood, kidney, and liver, and localizes primarily to nuclear speckles. Notably, PRPF3 undergoes phosphorylation, a post-translational modification that can influence function and interactions within the spliceosome. PRPF3 directly interacts with PRPF4 and is present in the inactive spliceosome, highlighting importance in the splicing process, particularly in photoreceptor-specific genes. Mutations in the gene encoding PRPF3 are linked to autosomal dominant retinitis pigmentosa type 18 (RP18), a condition characterized by progressive photoreceptor cell degeneration, initially manifesting as a loss of midperipheral vision and night blindness, ultimately leading to severe vision impairment. This underscores the critical role of PRPF3 in maintaining retinal health and potential as a target for therapeutic intervention in retinal diseases.

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PRPF3 Antibody (42-N) References:

1. Mutations in HPRP3, a third member of pre-mRNA splicing factor genes, implicated in autosomal dominant retinitis pigmentosa.  |  Chakarova, CF., et al. 2002. Hum Mol Genet. 11: 87-92. PMID: 11773002
2. Central region of the human splicing factor Hprp3p interacts with Hprp4p.  |  Gonzalez-Santos, JM., et al. 2002. J Biol Chem. 277: 23764-72. PMID: 11971898
3. Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.  |  Comitato, A., et al. 2007. Hum Mol Genet. 16: 1699-707. PMID: 17517693
4. Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex.  |  Gonzalez-Santos, JM., et al. 2008. Hum Mol Genet. 17: 225-39. PMID: 17932117
5. Hypoxia-regulated components of the U4/U6.U5 tri-small nuclear riboprotein complex: possible role in autosomal dominant retinitis pigmentosa.  |  Schmidt-Kastner, R., et al. 2008. Mol Vis. 14: 125-35. PMID: 18334927
6. The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p.  |  Lauber, J., et al. 1997. RNA. 3: 926-41. PMID: 9257651
7. Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome.  |  Wang, A., et al. 1997. Hum Mol Genet. 6: 2117-26. PMID: 9328476
8. A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs.  |  Horowitz, DS., et al. 1997. RNA. 3: 1374-87. PMID: 9404889

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRPF3 Antibody (42-N) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PRPF3 Antibody (42-N) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRPF3 Antibody (42-N) Specifications

Alternate Product Name: PRPF3

Product Clone Name: 42-N

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 585-684

Epitope Target: PRPF3

Epitope Species: human

Product Reactivity/Detection: PRPF3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PRPF3 Antibody (42-N): N/A