Date published: 2025-9-5
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PRMT1 Antibody (B-2) - Western Blotting - Image 129877
PRMT1 抗体 (B-2): sc-166963. COLO 320DM (A), HeLa (B), IMR-32 (C) 和 WI-38 (D) 全细胞裂解液中 PRMT1 表达的 Western 印迹分析.
PRMT1 抗体 (B-2): sc-166963. COLO 320DM (A), HeLa (B), IMR-32 (C) 和 WI-38 (D) 全细胞裂解液中 PRMT1 表达的 Western 印迹分析.

PRMT1 抗体 (B-2): sc-166963

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寫評論提問

说明书
  • PRMT1 抗体 B-2 是小鼠单克隆 IgG1 κ，PRMT1抗体, 在20篇文献中引用，规格为200 µg/ml
  • 针对氨基酸166-300的图谱，在PRMT1的内部区域，human起源
  • PRMT1 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PRMT1
  • 抗PRMT1抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PRMT1 (B-2): sc-166963的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PRMT1 抗体 (B-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PRMT1 抗体 (B-2) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 PRMT 抗体 包括 PRMT1, PRMT2, PRMT5, PRMT6和PRMT7

PRMT1抗体（B-2）是一种IgG1κ小鼠单克隆PRMT1抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的PRMT1蛋白。PRMT1抗体（B-2）可作为非偶联抗PRMT1抗体形式，以及多种偶联形式的抗PRMT1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。一类称为1型蛋白质精氨酸N-甲基转移酶（PRMTs）酶的蛋白质参与RNA结合蛋白的翻译后修饰，但在底物特异性、寡聚化特性和亚细胞定位方面有所不同。PRMT1是哺乳动物细胞中的主要形式，位于细胞核中。在羧基末端，白细胞介素增强因子结合因子3（ILF3）与PRMT1结合，从而调节PRMT1的活性。PRMT基因的替代mRNA剪接导致三种不同亚型的PRMT1，其氨基末端区域不同，都具有酶活性。PRMT8，也称为HRMT1L3或HRMT1L4（异质核核糖核蛋白甲基转移酶样蛋白4），是1型PRMT家族的一个独特成员，具有组织特异性表达和质膜定位。PRMT8在脑中特异性表达，在神经元分化中可能具有精氨酸甲基转移酶的作用。它与PRMT1最密切相关，可能是通过基因复制产生的。PRMT8可以与PRMT1异二聚化，具有相似的底物偏好。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PRMT1 抗体 (B-2) 参考文献:

  1. 骨骼肌特异性 Prmt1 基因缺失会通过 PRMT6-FOXO3 轴的失调导致肌肉萎缩。  |  Choi, S., et al. 2019. Autophagy. 15: 1069-1081. PMID: 30653406
  2. PRMT1对EZH2的甲基化调节其稳定性并促进乳腺癌转移。  |  Li, Z., et al. 2020. Cell Death Differ. 27: 3226-3242. PMID: 32895488
  3. PRMT1介导的H4R3me2a通过增强EGFR信号传导，招募SMARCA4促进结直肠癌的进展。  |  Yao, B., et al. 2021. Genome Med. 13: 58. PMID: 33853662
  4. PRMT1对PHGDH精氨酸的甲基化促进丝氨酸的合成，是肝癌治疗的一个薄弱环节。  |  Wang, K., et al. 2023. Nat Commun. 14: 1011. PMID: 36823188
  5. 靶向PRMT1介导的SRSF1甲基化抑制致癌外显子插入事件和乳腺癌的发生。  |  Li, WJ., et al. 2023. Cell Rep. 42: 113385. PMID: 37938975
  6. PRMT1抑制激活干扰素通路，增强黑色素瘤的抗肿瘤免疫并提高检查点阻断功效。  |  Tao, H., et al. 2024. Cancer Res. 84: 419-433. PMID: 37991725
  7. PRMT1通过甲基化PHGDH维持从头脂肪酸合成，从而驱动三阴性乳腺癌的耐药性。  |  Yamamoto, T., et al. 2024. Cancer Res. 84: 1065-1083. PMID: 38383964
  8. Git1-PGK1相互作用通过调节Keap1/Nrf2信号传导实现脊髓缺血再灌注损伤的自我保护。  |  Liu, H., et al. 2024. Cell Death Dis. 15: 170. PMID: 38402202
  9. PRMT1促进胰腺癌的发展和化疗耐药性。  |  Ku, B., et al. 2024. Cell Rep Med. 5: 101461. PMID: 38460517
  10. CRISPR筛选通过双层调节机制确定PRMT1为关键促铁死亡基因。  |  Zhang, X., et al. 2024. Cell Rep. 43: 114662. PMID: 39178116

订购信息

产品名称产品编号规格价格数量收藏夹

PRMT1 抗体 (B-2)

sc-166963
200 µg/ml
$316.00

PRMT1 (B-2): m-IgG Fc BP-HRP 套装

sc-527201
200 µg Ab; 10 µg BP
$354.00

PRMT1 (B-2): m-IgG1 BP-HRP 套装

sc-532574
200 µg Ab; 20 µg BP
$354.00

PRMT1 抗体 (B-2) AC

sc-166963 AC
500 µg/ml, 25% agarose
$416.00

PRMT1 抗体 (B-2) HRP

sc-166963 HRP
200 µg/ml
$316.00

PRMT1 抗体 (B-2) FITC

sc-166963 FITC
200 µg/ml
$330.00

PRMT1 抗体 (B-2) PE

sc-166963 PE
200 µg/ml
$343.00

PRMT1 抗体 (B-2) Alexa Fluor® 488

sc-166963 AF488
200 µg/ml
$357.00

PRMT1 抗体 (B-2) Alexa Fluor® 546

sc-166963 AF546
200 µg/ml
$357.00

PRMT1 抗体 (B-2) Alexa Fluor® 594

sc-166963 AF594
200 µg/ml
$357.00

PRMT1 抗体 (B-2) Alexa Fluor® 647

sc-166963 AF647
200 µg/ml
$357.00

PRMT1 抗体 (B-2) Alexa Fluor® 680

sc-166963 AF680
200 µg/ml
$357.00

PRMT1 抗体 (B-2) Alexa Fluor® 790

sc-166963 AF790
200 µg/ml
$357.00

I want to use this antibody to make a experiment of immunoprecipitation in the mouse preadipocytes，can you ensure you product can satisfy my requirement ? Or maybe I need to apply for a trial product.

Asked by: Anonymous
Thanks for your questions. Anti-PRMT1 Antibody (B-2) is recommended for detection of PRMT1 of mouse origin by IP. 感谢您的问题。Anti-PRMT1 抗体 (B-2)是推荐免疫沉淀方法检测小鼠来源样本的，该产品不质保免疫共沉淀。
Answered by: Technical Support 9
Date published: 2020-06-12

I am using PRMT1 (B-2): sc-166963 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from The sc-166963 antibody does not workI prepared 10µg of whole cell lysates of HuH7 or PLC cells and run them on a 10% SDS-PAGE gel. Proteins were transferred from the gel into a nitrocellulose membrane and after blocking, membranes were incubated with the respective antibody rocking them in a shaker at 4 degree over night. The membranes were then washed and incubated with the appropriate secondary antibody and developed with the LI-COR Odyssey imager. The antibody against PRMT1 did not properly work and it produced a not specific staining.
Date published: 2024-01-23
Rated 1 out of 5 by from This Ab did not work.I recently purchased this Ab and found it did not work for WB. There was no any band detected in my several cell lines and I did detected the PRMT1 bands using Cell Signaling PRMT1 Ab (Cat# 2449S, This Ab detected two bands in my cell lines. Therefore I tried to use Santa Cruz mAb). I asked for replacing it and found the replaced one did not work either.
Date published: 2022-09-02
Rated 5 out of 5 by from high-activeStrong bands were observed in human tissues by immunoblotting, and it was very effective for WB.
Date published: 2017-10-26
Rated 5 out of 5 by from good performanceI usually use this PRMT1 anitbody .This PRMT1 antibody worked very well for WB, it be diluted 1:1000,we got clear and strong signal at 42kda
Date published: 2017-09-02
Rated 5 out of 5 by from Excellent antibodya free sample, but a good result !! I will continue to support SCBT
Date published: 2017-08-15
Rated 4 out of 5 by from this antibody also can be used mouse ES cellsthis antibody also can be used mouse ES cells and can be detected about 35KDa.
Date published: 2017-06-27
Rated 5 out of 5 by from good result.This was used for IP detection in AGS, when diluted 1:1000 is a good signal.
Date published: 2017-06-21
Rated 5 out of 5 by from specific signalI am glad to buy this antibody. I used it to do western blot .It have signal in Hela cell line.
Date published: 2017-05-31
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PRMT1 抗体 (B-2) is rated 4.3 out of 5 by 12.
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