PRK2 Double Nickase Plasmid (h): sc-407649-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRK2 Double Nickase Plasmid (h) and PRK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PKN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRK2 Antibody (A-3): sc-271971
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRK2 Double Nickase Plasmid (h) | sc-407649-NIC | 20 µg | $410.00 | |||
PRK2 Double Nickase Plasmid (h2) | sc-407649-NIC-2 | 20 µg | $410.00 |
PKN2 encodes protein kinase N2 (PRK2), a serine/threonine kinase that functions as an effector of Rho family GTPases to coordinate cytoskeletal remodeling, actin–myosin contractility, and cell adhesion dynamics. PRK2 contributes to signaling modules that intersect with focal adhesion and stress fiber organization, influencing cell shape changes, migration, and cytokinesis. Through regulation of phosphorylation events in these pathways, altered PRK2 activity or expression is studied in contexts of aberrant motility, invasive phenotypes, and dysregulated growth signaling relevant to cancer and vascular biology. PRK2 is also investigated for roles in cellular stress responses and kinase network crosstalk that shape proliferation and survival programs.
PRK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PKN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PKN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PKN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PKN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.