PPARγ (h): 293T Lysate: sc-159760

PPARγ (h): 293T Lysate is a critical research tool derived from the human embryonic kidney 293T cell line, which has been engineered to express the human Peroxisome Proliferator-Activated Receptor Gamma (PPARγ). This nuclear receptor is pivotal in regulating gene expression linked to adipogenesis, lipid metabolism, and glucose homeostasis. The 293T cell line, renowned for its high transfection efficiency and robust protein expression, provides an ideal system for studying PPARγ's role in cellular processes. Mechanistically, PPARγ functions by forming heterodimers with retinoid X receptors (RXRs) and binding to PPAR response elements (PPREs) in the DNA, thereby modulating the transcription of target genes. Research applications of PPARγ (h): 293T Lysate include investigating the molecular pathways involved in metabolic regulation, dissecting the interactions between PPARγ and co-regulators, and screening for chemical modulators that influence PPARγ activity. Additionally, this lysate is instrumental in proteomic studies to identify PPARγ-associated proteins and in chromatin immunoprecipitation (ChIP) assays to map PPARγ binding sites on a genome-wide scale. The lysate's utility extends to elucidating the crosstalk between PPARγ signaling and other metabolic pathways, offering insights into fundamental biological processes.