Anti-PP2A-C alpha / beta Antibody (0.T.118) recommended for detection of PP2A-Cα/β of mouse, rat, human, Xenopus, zebrafish, bovine, porcine and canine origin by WB, IP and IF

Anti-PP2A-Cα/β Antibody (0.T.118): sc-56950

Anti-PP2A-Cα/β Antibody (0.T.118) is rated 5.0 out of 5 by 3.
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Datasheets

    • PP2A-Cα/β Antibody (0.T.118) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 100 µg/ml
    • raised against amino acids 295-309 of PP2A-Cα/β of human origin
    • recommended for detection of PP2A-Cα/β of mouse, rat, human, Xenopus, zebrafish, bovine, porcine and canine origin by WB, IP and IF; to demethylate, treat with 100 mM NaOH on ice
    • See PP2A-Cα/β (1D6): sc-80665 for PP2A-Cα/β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for PP2A-Cα/β Antibody (0.T.118) for WB applications. This reagent is now offered in a bundle with PP2A-Cα/β Antibody (0.T.118) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended starting dilution for Western blot for PP2A-Cα/β (0.T.118): sc-56950 monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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Rated 5 out of 5 by from Great WB data obtained using mouse PP2A Great WB data obtained using mouse PP2A-C transfected 293T, A-673 and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size in human PP2A-C transfected 293Tand MCF7 whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Good specificity and signal strength in KNRK Good specificity and signal strength in KNRK (A) and A-431 (B) whole cell lysates by WB. -SCBT QC
Date published: 2014-10-12
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