PP2A-Aα Antibody (5H4): sc-56952
- PP2A-Aα Antibody (5H4) is a rat monoclonal IgM provided at 200 µg/ml
- raised against the N-terminus of PP2A-Aα of human origin
- recommended for detection of PP2A-Aα of mouse, rat and human origin by WB and IP
- See PP2A-Aα (6G3): sc-56954 for PP2A-Aα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PP2A-Aα Antibody (5H4). This work is in progress.
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PP2A-Aα Antibody (5H4) is a rat monoclonal IgM antibody that detects PP2A-Aα in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). PP2A-Aα is a crucial regulatory subunit of the protein phosphatase 2A (PP2A) complex, which plays a vital role in cellular signaling pathways by dephosphorylating serine and threonine residues on target proteins. This dephosphorylation is essential for regulating various cellular functions, including cell division, apoptosis, and maintaining cellular homeostasis. The PP2A complex consists of a catalytic subunit and regulatory subunits, with PP2A-Aα serving as one of the key regulatory components that modulate catalytic subunit activity. PP2A-Aα interaction with associated proteins is critical for PP2A holoenzyme assembly and stability, influencing substrate specificity and overall function. Understanding PP2A-Aα dynamics and interactions helps elucidate signal transduction mechanisms and potential implications in various diseases, including cancer, where dysregulation of phosphatase activity can lead to aberrant cell growth and survival.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PP2A-Aα Antibody (5H4) References:
- Role of a novel functional variant in the PPP2R1A promoter on the regulation of PP2A-Aalpha and the risk of hepatocellular carcinoma. | Chen, HF., et al. 2013. PLoS One. 8: e59574. PMID: 23555712
- Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. | Jeong, AL., et al. 2016. Sci Rep. 6: 27391. PMID: 27272709
- The Highly Recurrent PP2A Aα-Subunit Mutation P179R Alters Protein Structure and Impairs PP2A Enzyme Function to Promote Endometrial Tumorigenesis. | Taylor, SE., et al. 2019. Cancer Res. 79: 4242-4257. PMID: 31142515
- A Cancer-Associated Missense Mutation in PP2A-Aα Increases Centrosome Clustering during Mitosis. | Antao, NV., et al. 2019. iScience. 19: 74-82. PMID: 31357169
- The broad phenotypic spectrum of PPP2R1A-related neurodevelopmental disorders correlates with the degree of biochemical dysfunction. | Lenaerts, L., et al. 2021. Genet Med. 23: 352-362. PMID: 33106617
- PPP2R1A neurodevelopmental disorder is associated with congenital heart defects. | Baker, EK., et al. 2022. Am J Med Genet A. 188: 3262-3277. PMID: 36209351
- Prenatal Diagnosis of PPP2R1A-Related Neurodevelopmental Disorders Using Whole Exome Sequencing: Clinical Report and Review of Literature. | Lei, T., et al. 2023. Genes (Basel). 14: PMID: 36672867
- PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex. | Wang, Y., et al. 2023. Nat Commun. 14: 3541. PMID: 37322026
- Novel Variants of PPP2R1A in Catalytic Subunit Binding Domain and Genotype-Phenotype Analysis in Neurodevelopmentally Delayed Patients. | Qian, Y., et al. 2023. Genes (Basel). 14: PMID: 37761890
- PPP2R1A silencing suppresses LUAD progression by sensitizing cells to nelfinavir-induced apoptosis and pyroptosis. | Liu, Y., et al. 2024. Cancer Cell Int. 24: 145. PMID: 38654331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP2A-Aα Antibody (5H4) | sc-56952 | 200 µg/ml | $322.00 |