RNAi-directed mRNA Cleavage
Pontin 52 siRNA and shRNA Plasmids (h)
- Target species: human
- CRISPR/Cas9 KO Plasmids consists of Pontin 52-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using Pontin 52 Antibody (A-11): sc-393905
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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siRNA
Primer
shRNA Plasmid
shRNA Lentiviral Particles
Recommended Control Antibody
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Pontin 52 Antibodies for analysis of cellular responses to Pontin 52 si/shRNA Gene Silencers
Description
- 10 µM, 50-100 transfections
- siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
- for independent verification of Pontin 52 gene silencing results, individual siRNA duplex components are also available upon request
- suitable control antibody: Pontin 52 Antibody (A-11): sc-393905
- sqRT-PCR Primer also available: Pontin 52 (h)-PR: sc-43543-PR, 20 µl
- Get details about si/shRNA Gene Silencers
See Protocol Online.
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Description
- 20 µl of each primer set is provided at a concentration of 10 µM
- Pontin 52 Primer (h) includes A set (forward & reverse) and B set (forward & reverse) primers
- Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR
See Protocol Online.
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Description
- 20 µg, up to 20 transfections
- shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
- shRNA sequences correspond to Pontin 52 siRNA Gene Silencer sequences
- provided as transfection-ready purified plasmid DNA target-specific gene silencing
- after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
- shRNA Plasmids are intended only for non-commercial use.
- suitable control antibody: Pontin 52 Antibody (A-11): sc-393905
- sqRT-PCR Primer also available: Pontin 52 (h)-PR: sc-43543-PR, 20 µl
See Protocol Online.
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Description
- 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
- Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
- Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
- shRNA sequences correspond to Pontin 52 siRNA Gene Silencer sequences
- After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
- Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
- Lentiviral Particles are intended for non-commercial use.
See Protocol Online.
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pontin 52 siRNA (h) | sc-43543 | 10 µM | $298.00 | |||
Pontin 52 (h)-PR | sc-43543-PR | 10 µM, 20 µl | $34.00 | |||
Pontin 52 shRNA Plasmid (h) | sc-43543-SH | 20 µg | $603.00 | |||
Pontin 52 shRNA (h) Lentiviral Particles | sc-43543-V | 200 µl | $725.00 |