Date published: 2025-10-12

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PNO1 Antibody (G-7): sc-514727

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Datasheets
  • PNO1 Antibody (G-7) is a mouse monoclonal IgM κ PNO1 antibody, cited in 2 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 170-192 within an internal region of PNO1 of human origin
  • recommended for detection of PNO1 of mouse, rat and human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PNO1 Antibody (G-7). This work is in progress.

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PNO1 Antibody (G-7) is a mouse monoclonal IgM antibody that detects PNO1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PNO1 antibody (G-7) is available as the non-conjugated form. PNO1, also known as KHRBP1, is a 252 amino acid protein primarily localized to the nucleolus, where PNO1 plays a crucial role in RNA binding events during transcription and translation. This nucleolar localization is significant for ribosome biogenesis and ribosomal RNA processing, which are vital for protein synthesis in the cell. PNO1 is expressed in various tissues, including kidney, lung, liver, and spleen, with lower expression levels in brain, heart, colon, and skeletal muscle. PNO1 maps to human chromosome 2, a region containing over 1,400 genes and comprising nearly 8% of the human genome. Chromosome 2 is associated with several genetic disorders, including Harlequin ichthyosis, linked to mutations in ABCA12 gene, and sitosterolemia, caused by defects in ABCG5 and ABCG8 genes. Mutations in ALMS1 gene, located on chromosome 2, are responsible for the rare recessive genetic disorder known as Alström syndrome.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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PNO1 Antibody (G-7) References:

  1. Cloning and characterization of a novel human RNA binding protein gene PNO1.  |  Zhou, GJ., et al. 2004. DNA Seq. 15: 219-24. PMID: 15497447
  2. Cloning, expression and characterization of the human NOB1 gene.  |  Zhang, Y., et al. 2005. Mol Biol Rep. 32: 185-9. PMID: 16172919
  3. ABCA12 is the major harlequin ichthyosis gene.  |  Thomas, AC., et al. 2006. J Invest Dermatol. 126: 2408-13. PMID: 16902423
  4. Spectrum of ALMS1 variants and evaluation of genotype-phenotype correlations in Alström syndrome.  |  Marshall, JD., et al. 2007. Hum Mutat. 28: 1114-23. PMID: 17594715
  5. Compound heterozygous ABCA12 mutations including a novel nonsense mutation underlie harlequin ichthyosis.  |  Akiyama, M., et al. 2007. Dermatology. 215: 155-9. PMID: 17684380
  6. Alström syndrome.  |  Marshall, JD., et al. 2007. Eur J Hum Genet. 15: 1193-202. PMID: 17940554
  7. Origin of human chromosome 2: an ancestral telomere-telomere fusion.  |  IJdo, JW., et al. 1991. Proc Natl Acad Sci U S A. 88: 9051-5. PMID: 1924367

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PNO1 Antibody (G-7)

sc-514727
200 µg/ml
$316.00

PNO1 (G-7) Neutralizing Peptide

sc-514727 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-514727 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great choice for Western Blot of PNO1This antibody detects over-expressed mouse PNO1 in sc-125837 and endogenous PNO1 in human cell lines K-562, HeLa, and RT-4. -SCBT QC
Date published: 2023-09-14
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PNO1 Antibody (G-7) is rated 5.0 out of 5 by 1.
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