Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PITSLRE CRISPR/Cas9 KO Plasmid (m): sc-419583
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PITSLRE CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PITSLRE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PITSLRE Antibody (B-9): sc-377296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PITSLRE CRISPR/Cas9 KO Plasmid (m) | sc-419583 | 20 µg | $397.00 |
Overview
Mouse Cdk11b encodes the PITSLRE serine/threonine kinase, a cyclin-dependent kinase family member implicated in coordinating cell-cycle progression with transcriptional and RNA-processing programs. PITSLRE activity has been linked to regulation of pre-mRNA splicing, mitotic control, and apoptosis, supporting proteostasis and genome integrity in proliferating cells. Disruption of PITSLRE-associated pathways can alter checkpoint responses and stress signaling, processes frequently studied in models of dysregulated proliferation and tissue homeostasis. As a node connecting kinase signaling to nuclear gene expression machinery, Cdk11b is relevant to mechanistic investigations of growth control and disease-associated cellular phenotypes in mouse systems.
PITSLRE CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cdk11b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cdk11b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cdk11b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PITSLRE protein expression.
This CRISPR knockout system enables efficient generation of Cdk11b-deficient cell models for investigation of PITSLRE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cdk11b exon(s) critical for PITSLRE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cdk11b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PITSLRE CRISPR/Cas9 KO Plasmid (m) and PITSLRE CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cdk11b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PITSLRE HDR Plasmid (m) and PITSLRE HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cdk11b homology arms to support homology-directed repair at defined Cdk11b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.