Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pin1 Double Nickase Plasmid (h): sc-400485-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pin1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Pin1 Double Nickase Plasmid (h) and Pin1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PIN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pin1 Antibody (G-8): sc-46660
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pin1 Double Nickase Plasmid (h) | sc-400485-NIC | 20 µg | $410.00 | |||
Pin1 Double Nickase Plasmid (h2) | sc-400485-NIC-2 | 20 µg | $410.00 |
Human PIN1 encodes Pin1, a phosphorylation-dependent peptidyl-prolyl cis/trans isomerase that recognizes Ser/Thr-Pro motifs and catalyzes conformational switches controlling protein stability, localization, and signaling output. Pin1 integrates inputs from proline-directed kinases and modulates pathways central to cell-cycle progression, transcriptional regulation, DNA damage responses, and stress signaling through substrates such as cyclin D1, c-Myc, p53, and tau. By reshaping phospho-protein conformations, Pin1 influences ubiquitin-mediated turnover and checkpoint control, linking phosphorylation dynamics to proteostasis. Dysregulated PIN1 activity has been associated with oncogenic signaling programs and neurodegenerative processes, making it a widely used node for mechanistic studies of phosphorylation-driven regulation.
Pin1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PIN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PIN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PIN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PIN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.