Per2 (h): 293T Lysate: sc-129449

Per2 (h): 293T Lysate, derived from the HEK 293T cell line, is a crucial tool for studying the Period Circadian Regulator 2 (Per2) protein in human cells. HEK 293T cells, a modified version of the original HEK 293 cells, express the SV40 large T antigen, which enhances their ability to produce high levels of recombinant proteins. This lysate is particularly valuable for investigating the molecular mechanisms underlying circadian rhythms. Researchers use Per2 (h): 293T Lysate to explore how Per2 regulates circadian rhythms by interacting with other core clock proteins, such as CLOCK, BMAL1, and CRY. Per2 is a key component of the feedback loop that generates circadian oscillations, influencing the transcriptional-translational feedback mechanism that drives the 24-hour cycle of gene expression. Techniques such as co-immunoprecipitation, Western blotting, and chromatin immunoprecipitation (ChIP) are commonly employed to study Per2's interactions and its role in the regulation of circadian genes. This lysate is instrumental in elucidating the post-translational modifications of Per2, such as phosphorylation, which affect its stability and function. By analyzing these modifications, scientists gain insights into the regulatory mechanisms that control circadian rhythms. Research using Per2 (h): 293T Lysate focuses strictly on fundamental biological processes, providing a deeper understanding of the molecular dynamics of circadian regulation. This research helps to advance our knowledge of cellular timekeeping mechanisms and their impact on physiological processes.