Date published: 2025-9-7
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PCNA Antibody (PC10) - Western Blotting - Image 399258
PCNA 항체(PC10): sc-56. HCT-116(A), Raji(B), HeLa(C), MOLT-4(D), NIH/3T3(E) 및 KNRK(F) 전체 세포 용해물에서 PCNA 발현의 형광 웨스턴 블롯 분석. UltraCruz® 차단 시약으로 차단: sc-516214. 사용된 검출 시약: m-IgG2a BP-CFL 647: sc-542738.
PCNA 항체(PC10): sc-56. HCT-116(A), Raji(B), HeLa(C), MOLT-4(D), NIH/3T3(E) 및 KNRK(F) 전체 세포 용해물에서 PCNA 발현의 형광 웨스턴 블롯 분석. UltraCruz® 차단 시약으로 차단: sc-516214. 사용된 검출 시약: m-IgG2a BP-CFL 647: sc-542738.

PCNA 항체 (PC10): sc-56

4.9(114)
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데이터 시트
  • PCNA (Clone PC10) is a mouse monoclonal IgG2a κ antibody, currently cited in 2,526 publications, and provided at 200 µg/ml
  • The Calculated Molecular Weight of PCNA is ~29 kDa, however observed weight of PCNA is ~36kDa.
  • Detects PCNA protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
  • PCNA (proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta that assists in DNA replication and DNA repair.
  • Antibodies for PCNA are antinuclear antibodies (ANAs) typically used to study cell proliferation, DNA replication, and cancer.
  • 항-PCNA 항체(PC10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • PCNA (PC10): sc-56의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

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PCNA 항체(PC10)는 WB, IP, IF, IHC(P) 및 FCM에 의해 m, r, h, 곤충, S. 폼베 기원의 PCNA 단백질을 검출하는 IgG2a κ 마우스 단일클론 PCNA 항체(또한 ATLD2 항체로 지정됨)이다. PCNA 항체(PC10)는 비- conjug 항-PCNA 항체 형태뿐만 아니라 아가로오스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 다중 접합 형태의 항-PCNA 항체로 이용 가능하다. PCNA(증식 세포 핵항원) 단백질은 세포 주기의 초기 G1 및 S 단계에서 합성되고, 세포 주기 진행, DNA 복제 및 DNA 복구 기능을 한다. 초기 S 단계에서, PCNA 단백질은 과립 분포를 나타내고 뉴클레오리에는 없지만, 후기 S 단계에서는 뉴클레오리로 재배치된다. 따라서, 염색 응용에서, PCNA 항체는 핵 염색을 나타낸다. PCNA는 두 가지 기본 형태로 존재하는데, 하나는 핵에 특이적으로 국한되는 진행 중인 DNA 복제에 관여하는 형태와, 일정한 합성에 관여하지 않는 두 번째 가용성 형태이다. 흥미롭게도, 후자의 형태는 유기 용매의 존재 하에서 분해되어 유기 고정제를 사용하는 조직에서 조직학적 방법으로 검출할 수 없게 만든다. 따라서, 합성 형태만이 이러한 방법을 사용하는 PCNA 항체에 의해 검출될 수 있다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

PCNA 항체 (PC10) 참고문헌:

  1. 초파리 PCNA 유전자 기능에 대한 E2F 조절 전사의 기여.  |  Thacker, SA., et al. 2003. Curr Biol. 13: 53-8. PMID: 12526745
  2. 초파리의 불일치 복구 및 PCNA 유전자에 대한 동형접합 및 이형접합 돌연변이의 미세 위성 위치에서 생식선 돌연변이.  |  Baida, A., et al. 2003. DNA Repair (Amst). 2: 827-33. PMID: 12826283
  3. 인간 증식 세포 핵 항원은 전사 활성제 p300의 활성을 조절하고 전사 억제를 촉진합니다.  |  Hong, R. and Chakravarti, D. 2003. J Biol Chem. 278: 44505-13. PMID: 12937166
  4. 아르마딜로/팬골린은 PCNA와 DREF 프로모터 활동을 조절합니다.  |  Kwon, E., et al. 2004. Biochim Biophys Acta. 1679: 256-62. PMID: 15358517
  5. 첫 번째 및 후속 세포 주기 동안 배아에서 S-상 마커로서의 GFP-PCNA.  |  Kisielewska, J., et al. 2005. Biol Cell. 97: 221-9. PMID: 15584900
  6. 초파리의 PCNA 돌연변이체에서 생식세포 게놈 불안정성: DNA 지문 분석 및 마이크로위성 분석.  |  López, A., et al. 2005. Mutat Res. 570: 253-65. PMID: 15708584
  7. 원발성 위장관 림프종에서 증식 세포 핵 항원(PCNA) 면역 염색의 평가와 조직학적 등급, S+G2+M 상 분획(유세포 분석) 및 예후와의 관계.  |  Woods, AL., et al. 1991. Histopathology. 19: 21-7. PMID: 1680784
  8. 증식하는 세포 핵 항원(PCNA)의 단일 클론 항체 분석. 구조 보존 및 핵 형태 검출.  |  Waseem, NH. and Lane, DP. 1990. J Cell Sci. 96 (Pt 1): 121-9. PMID: 1695635
  9. 고대의 PCNA 단백질.  |  Pan, M., et al. 2011. Biochem Soc Trans. 39: 20-4. PMID: 21265741
  10. 세포 주기 동안 사이클린/증식하는 세포 핵 항원의 두 가지 집단이 존재: DNA 복제 부위와의 연관성.  |  Bravo, R. and Macdonald-Bravo, H. 1987. J Cell Biol. 105: 1549-54. PMID: 2889739
  11. 인간 DNA 중합효소 δ에서 PCNA 상호 작용 단백질 모티프 확인.  |  Khandagale, P., et al. 2020. Biosci Rep. 40: PMID: 32314787
  12. 10nm 이하 형광 이미징을 위한 단백질 기반 나노룰러로서의 PCNA.  |  Helmerich, DA., et al. 2024. Adv Mater. 36: e2310104. PMID: 38009560

주문정보

제품명카탈로그 번호 단위가격수량관심품목

PCNA 항체 (PC10)

sc-56
200 µg/ml
$316.00

PCNA (PC10): m-IgG Fc BP-HRP 번들

sc-528093
200 µg Ab; 10 µg BP
$354.00

PCNA (PC10): m-IgGκ BP-HRP 번들

sc-520391
200 µg Ab, 40 µg BP
$354.00

PCNA (PC10): m-IgG2a BP-HRP 번들

sc-547021
200 µg Ab; 10 µg BP
$354.00

PCNA 항체 (PC10) AC

sc-56 AC
500 µg/ml, 25% agarose
$416.00

PCNA 항체 (PC10) HRP

sc-56 HRP
200 µg/ml
$316.00

PCNA 항체 (PC10) FITC

sc-56 FITC
200 µg/ml
$330.00

PCNA 항체 (PC10) PE

sc-56 PE
200 µg/ml
$343.00

PCNA 항체 (PC10) Alexa Fluor® 488

sc-56 AF488
200 µg/ml
$357.00

PCNA 항체 (PC10) Alexa Fluor® 546

sc-56 AF546
200 µg/ml
$357.00

PCNA 항체 (PC10) Alexa Fluor® 594

sc-56 AF594
200 µg/ml
$357.00

PCNA 항체 (PC10) Alexa Fluor® 647

sc-56 AF647
200 µg/ml
$357.00

PCNA 항체 (PC10) Alexa Fluor® 680

sc-56 AF680
200 µg/ml
$357.00

PCNA 항체 (PC10) Alexa Fluor® 790

sc-56 AF790
200 µg/ml
$357.00

PCNA 항체 (PC10) B

sc-56 B
200 µg/ml
$320.00

PCNA 항체 (PC10) TRITC

sc-56 TRITC
200 µg/ml
$320.00

PCNA 항체 (PC10) Alexa Fluor® 405

sc-56 AF405
200 µg/ml
$357.00

Is it necessary to perform an antigen unmasking when using the anti-PCNA (PC10) mAb antibody? If yes, what is the protocol? Thanks  

Asked by: JPS44
Thank you for your question. You can find our recommended protocol with instructions on how to perform antigen unmasking under this link https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2024-11-22

What blocking procedure to you recommend for using this mouse monoclonal antibody on mouse tissue?

Asked by: Bscience
Thank you for your question. Incubate specimens for 30 minutes with 10% normal blocking serum in PBS .https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-03-28

does antibody m-IgGκ BP-HRP:(sc-516102) compatible to using as secondary antibody against Anti-PCNA Antibody (PC10): sc-56 for western blot

Asked by: Anonymous
Thank you for your question. Anti-PCNA Antibody (PC10) is a mouse monoclonal IgG2a κ PCNA antibody, so it is compatible with m-IgGκ BP-HRP:(sc-516102), which recognizes kappa light chain mouse IgG, IgA and IgM antibodies.
Answered by: Tech Support Europe
Date published: 2023-04-15

Recently we observed that the PCNA sc-56 antibody in the tube is precipitated. It was stored at 4 degrees as recommended. Can the antibody be used for experimental purpose? If yes how do we use it? any recommendation from company's side?

Asked by: Anonymous
Thank you for your question. Please contact technical service directly so they can look into this matter.
Answered by: Tech Support Europe
Date published: 2021-01-18

Which antigen retrieval buffer should be used with PCNA sc-56, citrate or Tris? Can you please include this information in the product datasheet so that it becomes convenient for the user to begin with.

Asked by: Sobia
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this PCNA antibody. The detailed method can be found in our recommended protocol online.
Answered by: Technical Support
Date published: 2025-01-19

Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?

Asked by: xgtian
Thank you for your question. We have not tested this antibody on swine or pig tissue, however, we are aware of a few publications citing use of this antibody in pig samples. For example, PMID: 26389662.
Answered by: Technical Support
Date published: 2025-02-26

We are new to IHC. Thinking of using this antibody, PCNA Antibody: sc-56on our chicken samples. Wondering if there are any preferences on the conjugate for the secondary antibody, such as HRP, biotin etc. We don't need fluorescence

Asked by: SDas
Thank you for your question. This antibody has been previously cited for detection of PCNA in chicken samples. For IHC, you could use the unconjugated antibody, with an appropriate secondary antibody for detection, or the Biotin conjugated primary with an ABC Staining System, or the HRP conjugate for direct immunoperoxidase staining. Using the directly conjugated primary antibody can save you time and money on reagents. We do not have a preference for this particular antibody, and think you would be successful using either version of the antibody, depending on your preferences in your lab.
Answered by: Technical Support
Date published: 2023-04-15

PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?

Asked by: Anukana
Thank you for your question. This is provided as 200 ug in 1 mL.
Answered by: Technical Support
Date published: 2025-01-20
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Rated 5 out of 5 by from SC-56 works for rabbitsSC-56 works for rabbits. IHC(P): sections were redyhrated and incubated in sodium citrate buffer (pH6.0) at 85 degree for 30 min for antigen retrieval. Antibody was diluted in 2% goat serum and 1% BSA (1:500).
Date published: 2024-05-15
Rated 5 out of 5 by from Very good antibodyThe antibody works very well in western blot using 30ug of protein
Date published: 2024-04-05
Rated 5 out of 5 by from very good despite low dilution needed1:100 dilution Antigen retrieval: Citrate buffer (pH 6.0) in a microwave
Date published: 2024-02-12
Rated 5 out of 5 by from IHC效果很好strong and specifical signals in IHC-P experiments，staining mouse tissues.
Date published: 2022-11-20
Rated 5 out of 5 by from Works great for IF with super-resolution imagingI got the Alexa-488 conjugated antibody and have used to for IF on U-2 OS cells. There was nuclear specific PCNA labelling. I imaged the samples with dSTORM and SIM methods and the immunolabeling looks great.
Date published: 2021-07-29
Rated 5 out of 5 by from Works very well!We blotted several times with this antibody and it works perfectly!
Date published: 2021-06-16
Rated 5 out of 5 by from PCNA Ab for WB and IFExcellent antibody for WB and IF (fixed with paraformaldehyde) dilution 1:2000 Good housekeeping protein for human colon cancer cell lines
Date published: 2021-03-23
Rated 5 out of 5 by from Excellent antibodyExcellent bands with high dilution and no background
Date published: 2021-03-17
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PCNA 항체 (PC10) is rated 4.9 out of 5 by 114.
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