Pax-5 Antibody (1H9): sc-130922
- Pax-5 Antibody (1H9) is a rat monoclonal IgG2a provided at 200 µg/ml
- raised against a recombinant protein corresponding to amino acids 154-284 of Pax5 of mouse origin
- recommended for detection of Pax-5 of mouse, rat and human origin by WB, IP, IF and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See Pax-5 (A-11): sc-13146 for Pax-5 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Pax-5 Antibody (1H9). This work is in progress.
Pax-5 Antibody (1H9) is a rat monoclonal IgG2a antibody that detects Pax-5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Pax-5 is a crucial transcription factor that plays a significant role in B cell development, specifically regulating the differentiation of pro-B, pre-B, and mature B lymphocytes while being absent in plasma cells. Pax-5 influences the balance between immunoglobulin secretion and B cell proliferation, which is vital for maintaining a healthy immune response. Dysregulation of Pax-5, particularly through overexpression, has been associated with various hematological malignancies, including small lymphocytic lymphomas, where a specific translocation involving the PAX5 gene can lead to aberrant expression. Anti-Pax-5 antibody (1H9) serves as an essential tool for researchers studying B cell biology and the underlying mechanisms of related diseases, providing insights into the complex signaling pathways that govern immune cell function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pax-5 Antibody (1H9) References:
- Role of Pax-5 in the regulation of a mid-hindbrain organizer's activity. | Funahashi, J., et al. 1999. Dev Growth Differ. 41: 59-72. PMID: 10445503
- Pax-5 expression in nonhematopoietic tissues. | Torlakovic, E., et al. 2006. Am J Clin Pathol. 126: 798-804. PMID: 17050077
- Pax5 maintains cellular identity by repressing gene expression throughout B cell differentiation. | Carotta, S., et al. 2006. Cell Cycle. 5: 2452-6. PMID: 17102626
- Diagnostic uses of Pax5 immunohistochemistry. | Feldman, AL. and Dogan, A. 2007. Adv Anat Pathol. 14: 323-34. PMID: 17717432
- The role of Pax5 in leukemia: diagnosis and prognosis significance. | Shahjahani, M., et al. 2015. Med Oncol. 32: 360. PMID: 25428382
- Pax-5 Inhibits NF-κB Activity in Breast Cancer Cells Through IKKε and miRNA-155 Effectors. | Harquail, J., et al. 2018. J Mammary Gland Biol Neoplasia. 23: 177-187. PMID: 30032344
- Activating PAX gene family paralogs to complement PAX5 leukemia driver mutations. | Hart, MR., et al. 2018. PLoS Genet. 14: e1007642. PMID: 30216339
- Expression of PAX-5 in B Cell Hodgkin and Non Hodgkin Lymphoma. | Khan, MR., et al. 2018. Asian Pac J Cancer Prev. 19: 3463-3466. PMID: 30583670
- miRNAs 484 and 210 regulate Pax-5 expression and function in breast cancer cells. | Harquail, J., et al. 2019. Carcinogenesis. 40: 1010-1020. PMID: 30605519
- Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers. | Bhattacharya, S., et al. 2024. Cell Death Dis. 15: 617. PMID: 39183332
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-5 Antibody (1H9) | sc-130922 | 200 µg/ml | $316.00 | |||
Pax-5 Antibody (1H9) FITC | sc-130922 FITC | 200 µg/ml | $330.00 | |||
Pax-5 Antibody (1H9) PE | sc-130922 PE | 200 µg/ml | $343.00 |