Pax-1 CRISPR/Cas9 KO Plasmid (m): sc-422114
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pax-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pax-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-1 CRISPR/Cas9 KO Plasmid (m) | sc-422114 | 20 µg | $397.00 |
Overview
Mouse Pax1 encodes the paired box transcription factor Pax-1, a nuclear DNA-binding protein that regulates lineage specification and patterning during embryogenesis, particularly within paraxial mesoderm and the developing vertebral column. Pax-1 integrates developmental signaling inputs to control transcriptional programs involved in chondrogenesis, sclerotome differentiation, and segmental organization of the axial skeleton. Altered Pax1 function has been linked to congenital vertebral and skeletal malformations and is used as a marker and regulator in studies of spine formation and musculoskeletal development. In cellular models, Pax-1 activity is investigated for its effects on transcriptional networks governing proliferation and differentiation in mesenchymal and cartilage progenitor contexts.
Pax-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pax1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pax1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pax1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pax-1 protein expression.
This CRISPR knockout system enables efficient generation of Pax1-deficient cell models for investigation of Pax-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pax1 exon(s) critical for Pax-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pax1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pax-1 CRISPR/Cas9 KO Plasmid (m) and Pax-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pax1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pax-1 HDR Plasmid (m) and Pax-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pax1 homology arms to support homology-directed repair at defined Pax1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.