Anti-pan CEA Antibody (H-8) recommended for detection of pan CEA of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-pan CEA Antibody (H-8): sc-48364

Anti-pan CEA Antibody (H-8) is rated 4.8 out of 5 by 9.
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Datasheets

    • Anti-pan CEA Antibody (H-8) is a mouse monoclonal IgG1 κ pan CEA antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 35-334 mapping near the N-terminus of CEA (carcinoembryonic antigen) of human origin
    • pan CEA Antibody (H-8) is recommended for detection of pan CEA of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-pan CEA Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of pan CEA (H-8): sc-48364.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for pan CEA Antibody (H-8). This work is in progress.
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I am using pan CEA (H-8): sc-48364 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Easy staining! Human FFPE abnormal and normal tissues work well. Using sodium citrate for 30 minutes at 1:100.
Date published: 2017-11-17
Rated 4 out of 5 by from GOOD ANTIDODY! The pan CEA Antibody (H-8): sc-48364 provides light staining pattern for WB, the sample can be much better.
Date published: 2017-08-15
Rated 5 out of 5 by from strong band Strong single band in WB in mouse kidney tissue ,good specificity at dilution 1:400.
Date published: 2017-05-27
Rated 5 out of 5 by from good IF result Used in IF and good decent staining in mouse brain cells.
Date published: 2017-05-25
Rated 4 out of 5 by from great product This antibody works very well in ovarian cancer cells.I bought this a month ago and am so happy that I did pefect results use this antibody for western blotting.The dilution that I used was 1:500 and it's specific
Date published: 2017-05-18
Rated 5 out of 5 by from This antibody works well The use of this antibody was very good both in WB and IHC .
Date published: 2017-02-06
Rated 5 out of 5 by from Western blot dilution 1:500 effect is perfect I bought this antibody a month ago, I used this antibody Western blot dilution 1:500 do ovarian cancer cells are very good results.
Date published: 2017-02-04
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and membrane Superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-06
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