Anti-PACT Antibody (2830C1a) recommended for detection of PACT of human origin by WB and IP

Anti-PACT Antibody (2830C1a): sc-81569

Anti-PACT Antibody (2830C1a) is rated 5.0 out of 5 by 2.
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Datasheets

    • PACT Antibody (2830C1a) is a mouse monoclonal IgG1, cited in 2 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of PACT of human origin
    • recommended for detection of PACT of human origin by WB and IP
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PACT Antibody (2830C1a). This work is in progress.
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I am using PACT (2830C1a): sc-81569 antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Publishable IP and WB data with human transfected Publishable IP and WB data with human transfected HEK293 cells - SCBT Publication Review
Date published: 2015-01-23
Rated 5 out of 5 by from IP and WB publishable data with human and IP and WB publishable data with human and african green monkey HEK293 and Vero E6 cells - SCBT Publication Review
Date published: 2014-10-20
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