p53 Antibody (3H2821): sc-71815

p53 Antibody (3H2821) is an IgG1 kappa light chain mouse monoclonal antibody raised against the SVA31E7 mouse SV40-transformed cell line, detects wild-type p53 in mouse cells and is validated for western blotting, immunoprecipitation, and immunofluorescence. p53 mouse monoclonal antibody (3H2821) recognizes an epitope in the wild-type mouse p53 protein and is ideal for analyzing p53 levels in tissue lysates, isolating p53 protein complexes, and visualizing subcellular p53 localization. Anti-p53 monoclonal antibody (3H2821) is widely used in studying p53 pathways, DNA damage response, and tumor development in mouse models. The p53 protein, encoded by the Trp53 gene, is a key tumor suppressor responsible for maintaining genomic integrity. It acts as a transcription factor regulating genes involved in cell cycle arrest, DNA repair, apoptosis, and senescence. Structurally and functionally similar to human p53, mouse p53 plays a crucial role in cancer research and developmental biology, making it an essential model for studying p53-related pathways. The p53 protein comprises several important domains, including the Transactivation Domain (TAD) for gene expression, the DNA-Binding Domain (DBD) for regulating target genes, the Oligomerization Domain (OD) for tetramer formation, and the Regulatory Domain for post-translational modifications like phosphorylation and acetylation. Mouse models expressing wild-type or mutant p53 are widely used to explore tumor suppression mechanisms, DNA damage responses, and the development of cancer therapies. Due to the conservation of p53 pathways between mice and humans, research on mouse p53 has strong translational potential, leading to insights applicable to clinical practice. Monoclonal antibodies are valuable for detecting p53 in experimental assays. Santa Cruz Biotechnology provides a comprehensive selection of monoclonal antibodies targeting mouse, rat and human p53. Notable antibodies include p53 antibody (DO-1): sc-126, which targets the N-terminal region of p53 for detecting full-length protein and assessing p53 activation. These antibodies offer high specificity and are validated for western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and flow cytometry, providing reliable tools for p53 research. The p53 protein is fundamental for understanding tumor suppression and cellular stress responses. Monoclonal antibodies like the anti-p53 antibody (3H2821), and those from Santa Cruz Biotechnology, enable precise detection and analysis of p53 in cancer biology, drug discovery, and developmental studies. With these tools, researchers can further investigate p53’s functions and its role in cancer therapy development.

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p53 Antibody (3H2821) References:

1. Prognostic and predictive significance of p53 mutation in aggressive B-cell lymphoma.  |  Ichikawa, A. 2000. Int J Hematol. 71: 211-20. PMID: 10846825
2. Post-translational modifications and activation of p53 by genotoxic stresses.  |  Appella, E. and Anderson, CW. 2001. Eur J Biochem. 268: 2764-72. PMID: 11358490
3. Expression of p53 protein in pituitary adenomas.  |  Oliveira, MC., et al. 2002. Braz J Med Biol Res. 35: 561-5. PMID: 12011941
4. Central and carboxy-terminal regions of human p53 protein are essential for interaction and complex formation with PARP-1.  |  Wesierska-Gadek, J., et al. 2003. J Cell Biochem. 89: 220-32. PMID: 12704785
5. GEP associates with wild-type p53 in hepatocellular carcinoma.  |  Cheung, ST., et al. 2006. Oncol Rep. 15: 1507-11. PMID: 16685387
6. Enhanced expression of p53 protein in pityriasis rubra pilaris.  |  Baran, W., et al. 2006. Acta Derm Venereol. 86: 276-7. PMID: 16710602
7. [Transcription factor p53 and skin aging].  |  Gritsenko, DA., et al. 2017. Adv Gerontol. 30: 10-16. PMID: 28557384
8. The clinical significance of p53 aberrations in human tumours.  |  Bosari, S. and Viale, G. 1995. Virchows Arch. 427: 229-41. PMID: 7496591
9. p53 gene status in endometrial carcinomas showing diffuse positivity for p53 protein by immunohistochemical analysis.  |  Ambros, RA., et al. 1995. Mod Pathol. 8: 441-5. PMID: 7567946
10. A comparison of the biological activities of wild-type and mutant p53.  |  Zambetti, GP. and Levine, AJ. 1993. FASEB J. 7: 855-65. PMID: 8344485

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.