Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p300 Double Nickase Plasmid (m): sc-435902-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p300 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p300 Double Nickase Plasmid (m) and p300 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ep300. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p300 Antibody (F-4): sc-48343
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p300 Double Nickase Plasmid (m) | sc-435902-NIC | 20 µg | $410.00 | |||
p300 Double Nickase Plasmid (m2) | sc-435902-NIC-2 | 20 µg | $410.00 |
Mouse Ep300 encodes p300, a broadly acting lysine acetyltransferase and transcriptional coactivator that acetylates histones and non-histone substrates to shape chromatin accessibility and gene expression programs. p300 integrates signals from multiple transcription factors and contributes to enhancer function, cell-cycle control, DNA damage responses, and lineage-specific differentiation. Through modulation of acetylation-dependent transcriptional networks, p300 influences pathways such as p53-regulated stress responses, TGF-β/SMAD signaling, and inflammatory gene regulation. Dysregulated EP300 activity has been associated with altered epigenetic states and transcriptional remodeling observed across diverse disease-relevant contexts, including cancer biology, developmental disorders, and immune dysfunction.
p300 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ep300 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ep300. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ep300 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ep300-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.