p22-phox Antibody (44.1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against p22-phox of human origin, with epitope mapping to amino acids 29-33 and 182-188
Anti-p22-phox Antibody (44.1) is recommended for detection of p22-phox of mouse, rat and human origin by WB, IP and FCM
Anti-p22-phox Antibody (44.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p22-phox (44.1): sc-130550.
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Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
You state that this clone can recognize mouse p22. Do you have any example immunoblot or flow cytometry data for this antibody on mouse cells?
Asked by: David M
Thank you for your question. We have not yet tested this mouse monoclonal antibody, p22-phox (44.1): sc-130550, in mouse or rat samples. However, the antibody is expected to cross-react with mouse and rat based on sequence identity with the antigen used to develop the antibody. We do warranty the antibody for detection of p22-phox of mouse, rat or human origin.
Answered by: Technical Support
Date published: 2018-01-12
Can p22-phox (44.1): sc-130550 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p22-phox (44.1): sc-130550 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 5 out of
Perfect for FACS applicationWe bought the sc-130550 PE (i.e. the PE coupled clone 44.1) and tested it on both PBMCs from a normal donor or from a p22phox mutated patient.
The signal is absolutely perfect and specific since only the control shows a nice and strong peak.
Date published: 2017-04-06
Rated 4 out of
Published WB data with human and hamster H661Published WB data with human and hamster H661, CHO-91-47-67, CHO-K1 - SCBT Publication Review
Date published: 2015-01-26
Rated 5 out of
Robust band present in western blot analysisRobust band present in western blot analysis of p22-phox expression in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-01-20
Rated 4 out of
Great intracellular FCM data of fixed andGreat intracellular FCM data of fixed and permeabilized THP-1 cells. -SCBT QC
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