p22-phox Antibody (44.1): sc-130550

p22-phox Antibody (44.1) is a mouse monoclonal IgG2a kappa light chain antibody that targets the p22-phox protein, also known as CYBA or cytochrome b-245 alpha chain, from human origin. anti-p22-phox antibody (44.1) recognizes specific epitopes at amino acids 29-33 and 182-188, enabling detection of p22-phox in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). p22-phox is an essential component of the NADPH oxidase complex, which plays a critical role in generating superoxide radicals in phagocytes, neuroepithelial cells, vascular smooth muscle cells, and endothelial cells. Together with gp91-phox (NOX2), p22-phox forms the membrane-bound flavocytochrome b558, necessary for the assembly and activation of the enzyme complex. During activation, cytosolic proteins p47-phox and p67-phox translocate to the plasma membrane to associate with p22-phox and gp91-phox, facilitating the production of reactive oxygen species that are vital for host defense and cellular signaling. Additionally, p22-phox acts as a surface oxygen sensor, initiating signaling pathways in response to hypoxic conditions. p22-phox monoclonal antibody (44.1) is available in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options for diverse experimental applications. anti-p22-phox antibody (44.1) supports the investigation of critical pathways involved in cellular signaling and the body′s defense mechanisms against oxidative damage.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p22-phox Antibody (44.1) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-p22-phox Antibody (44.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p22-phox Antibody (44.1) Specifications

Alternate Product Name: CYBA

Product Clone Name: 44.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 29-33 and 182-188

Epitope Target: p22-phox

Epitope Species: human

Product Reactivity/Detection: p22-phox

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for p22-phox Antibody (44.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p22-phox antibody is p22-phox Antibody (E-11): sc-271968.