p22-phox Antibody (44.1) recommended for detection of p22-phox of mouse, rat and human origin by WB, IP and FCM

p22-phox Antibody (44.1): sc-130550

p22-phox Antibody (44.1) is rated 4.5 out of 5 by 4.
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Datasheets

    • p22-phox Antibody (44.1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against p22-phox of human origin, with epitope mapping to amino acids 29-33 and 182-188
    • Anti-p22-phox Antibody (44.1) is recommended for detection of p22-phox of mouse, rat and human origin by WB, IP and FCM
    • Anti-p22-phox Antibody (44.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p22-phox (44.1): sc-130550.

Hello, You state that this clone can recognize mouse p22. Do you have any example immunoblot or flow cytometry data for this antibody on mouse cells? Thanks, David

Asked by: David M
Thank you for your question. We have not yet tested this mouse monoclonal antibody, p22-phox (44.1): sc-130550, in mouse or rat samples. However, the antibody is expected to cross-react with mouse and rat based on sequence identity with the antigen used to develop the antibody. We do warranty the antibody for detection of p22-phox of mouse, rat or human origin.
Answered by: Technical Support
Date published: 2018-01-12

Can p22-phox (44.1): sc-130550 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p22-phox (44.1): sc-130550 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect for FACS application We bought the sc-130550 PE (i.e. the PE coupled clone 44.1) and tested it on both PBMCs from a normal donor or from a p22phox mutated patient. The signal is absolutely perfect and specific since only the control shows a nice and strong peak.
Date published: 2017-04-06
Rated 4 out of 5 by from Published WB data with human and hamster H661 Published WB data with human and hamster H661, CHO-91-47-67, CHO-K1 - SCBT Publication Review
Date published: 2015-01-26
Rated 5 out of 5 by from Robust band present in western blot analysis Robust band present in western blot analysis of p22-phox expression in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-01-20
Rated 4 out of 5 by from Great intracellular FCM data of fixed and Great intracellular FCM data of fixed and permeabilized THP-1 cells. -SCBT QC
Date published: 2013-12-04
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