p-p53 Antibody (B-12): sc-377564

p-p53 Antibody (B-12) is a mouse monoclonal IgG1 antibody that detects p53 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p53 antibody (B-12) recognizes phosphorylated forms of p53, a crucial tumor suppressor protein that plays a vital role in cellular stress responses by regulating the expression of genes involved in growth arrest and apoptosis. p53 monoclonal antibody (B-12) targets p53 primarily located in the nucleus, where p53 functions as a transcription factor to maintain genomic stability and prevent tumorigenesis. Nuclear translocation to the cytoplasm, often mediated by the negative regulator MDM2, highlights the importance of nuclear localization in controlling cell cycle progression and apoptosis. MDM2, an E3 ubiquitin ligase, can polyubiquitinate p53, leading to degradation, which underscores the delicate balance between p53 activity and cellular homeostasis. Interactions with proteins such as 14-3-3σ enhance p53′s stability and nuclear retention by recognizing phosphorylated serine residues, while the acetyltransferase p300 further activates p53 by acetylating lysine residues, enabling stronger DNA binding and transcriptional activity. Furthermore, phosphorylation at specific residues, such as Thr 155, can promote interactions with DNA repair proteins, including BRCA1 and Rad51, emphasizing p53′s role in genomic stability. These complex networks of interactions demonstrate how post-translational modifications like phosphorylation fine-tune p53′s activity and interactions, making p53 (B-12) monoclonal antibody an invaluable tool for studying the dynamics of p53 regulation in cancer research and therapeutic development.

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.