p-Nitrophenyl 2-Acetamido-2-deoxy-3,4,6-tri-O-acetyl-α-D-glucopyranoside (CAS 13089-23-1)

p-Nitrophenyl 2-Acetamido-2-deoxy-3,4,6-tri-O-acetyl-α-D-glucopyranoside serves as a substrate analog frequently employed in biochemical and enzymological studies, particularly in investigations concerning glycoside hydrolases. This compound mimics the natural substrate of various glycosidases, including β-glucosidases and β-N-acetylhexosaminidases, facilitating the characterization of their catalytic properties and substrate specificity. Its mechanism of action involves the hydrolysis of the glycosidic bond by glycosidases, leading to the release of p-nitrophenol as a chromogenic product. Researchers utilize spectrophotometric assays to monitor the rate of p-nitrophenol formation, enabling the measurement of enzyme activity and determination of kinetic parameters. Moreover, p-Nitrophenyl 2-Acetamido-2-deoxy-3,4,6-tri-O-acetyl-α-D-glucopyranoside is employed in screening assays to identify potential inhibitors or activators of glycosidases, offering insights into their roles in biological processes and disease pathogenesis. By elucidating the biochemical properties and functions of glycosidases, this compound contributes to advancing our understanding of carbohydrate metabolism and the development of novel strategies for intervention.