Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OSC CRISPR/Cas9 KO Plasmid (m): sc-421475
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OSC CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OSC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OSC Antibody (D-6): sc-514507
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OSC CRISPR/Cas9 KO Plasmid (m) | sc-421475 | 20 µg | $397.00 |
Overview
Lss encodes oxidosqualene cyclase (OSC), a key endoplasmic reticulum–associated enzyme that cyclizes 2,3-oxidosqualene to lanosterol, committing flux into the sterol branch of the mevalonate pathway. Through control of sterol biosynthesis, OSC influences membrane cholesterol composition, lipid raft organization, and downstream steroidogenic capacity. Perturbation of sterol metabolism is broadly linked to dysregulated lipid homeostasis and cellular stress programs, making Lss a useful node for studying metabolic remodeling in physiology and disease-relevant contexts. In mouse models, Lss/OSC disruption provides a tractable approach to interrogate how sterol pathway constraints affect cell growth, differentiation, and signaling networks sensitive to membrane lipid composition.
OSC CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lss gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lss together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lss open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OSC protein expression.
This CRISPR knockout system enables efficient generation of Lss-deficient cell models for investigation of OSC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lss exon(s) critical for OSC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lss genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OSC CRISPR/Cas9 KO Plasmid (m) and OSC CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lss locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OSC HDR Plasmid (m) and OSC HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lss homology arms to support homology-directed repair at defined Lss target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.