ORP-1 Antibody (B-3): sc-376602
- ORP-1 Antibody (B-3) is a mouse monoclonal IgG2b κ ORP-1 antibody provided at 200 µg/ml
- raised against amino acids 898-950 mapping at the C-terminus of ORP-1 of human origin
- ORP-1 Antibody (B-3) is recommended for detection of ORP-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ORP-1 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for ORP-1 Antibody (B-3) for WB applications. This reagent is now offered in a bundle with ORP-1 Antibody (B-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
ORP-1 Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects ORP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ORP-1 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORP-1, a member of the OSBP (oxysterol-binding protein) family, plays a crucial role in lipid metabolism and cellular signaling, particularly in lipid transport within cells. ORP-1 is primarily located in the endoplasmic reticulum and can translocate to the Golgi apparatus in response to oxysterol binding, which is vital for maintaining cellular lipid homeostasis and facilitating vesicle transport. ORP-1 features a highly conserved OSBP-type sterol-binding region and a pleckstrin homology domain, allowing interactions with various lipids, including phosphatidic acid and phosphatidylinositol 3-phosphate. Three isoforms of ORP-1 arise from alternative splicing, with ORP-1A being specifically expressed in the retina, brain, and other select tissues, while ORP-1B is more ubiquitously expressed, highlighting ORP-1′s diverse functional roles across different biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ORP-1 Antibody (B-3) References:
- Family of human oxysterol binding protein (OSBP) homologues. A novel member implicated in brain sterol metabolism. | Laitinen, S., et al. 1999. J Lipid Res. 40: 2204-11. PMID: 10588946
- Novel members of the human oxysterol-binding protein family bind phospholipids and regulate vesicle transport. | Xu, Y., et al. 2001. J Biol Chem. 276: 18407-14. PMID: 11279184
- The OSBP-related protein family in humans. | Lehto, M., et al. 2001. J Lipid Res. 42: 1203-13. PMID: 11483621
- A family of 12 human genes containing oxysterol-binding domains. | Jaworski, CJ., et al. 2001. Genomics. 78: 185-96. PMID: 11735225
- Identification and assessment of the role of a nominal phospholipid binding region of ORP1S (oxysterol-binding-protein-related protein 1 short) in the regulation of vesicular transport. | Fairn, GD. and McMaster, CR. 2005. Biochem J. 387: 889-96. PMID: 15617515
- Oxysterol-binding protein and vesicle-associated membrane protein-associated protein are required for sterol-dependent activation of the ceramide transport protein. | Perry, RJ. and Ridgway, ND. 2006. Mol Biol Cell. 17: 2604-16. PMID: 16571669
- The OSBP-related proteins (ORPs): global sterol sensors for co-ordination of cellular lipid metabolism, membrane trafficking and signalling processes? | Olkkonen, VM., et al. 2006. Biochem Soc Trans. 34: 389-91. PMID: 16709169
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORP-1 Antibody (B-3) | sc-376602 | 200 µg/ml | $316.00 | |||
ORP-1 Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-523023 | 200 µg Ab, 40 µg BP | $354.00 | |||
ORP-1 Antibody (B-3) AC | sc-376602 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ORP-1 Antibody (B-3) HRP | sc-376602 HRP | 200 µg/ml | $316.00 | |||
ORP-1 Antibody (B-3) FITC | sc-376602 FITC | 200 µg/ml | $330.00 | |||
ORP-1 Antibody (B-3) PE | sc-376602 PE | 200 µg/ml | $343.00 | |||
ORP-1 Antibody (B-3) Alexa Fluor® 488 | sc-376602 AF488 | 200 µg/ml | $357.00 | |||
ORP-1 Antibody (B-3) Alexa Fluor® 546 | sc-376602 AF546 | 200 µg/ml | $357.00 | |||
ORP-1 Antibody (B-3) Alexa Fluor® 594 | sc-376602 AF594 | 200 µg/ml | $357.00 | |||
ORP-1 Antibody (B-3) Alexa Fluor® 647 | sc-376602 AF647 | 200 µg/ml | $357.00 | |||
ORP-1 Antibody (B-3) Alexa Fluor® 680 | sc-376602 AF680 | 200 µg/ml | $357.00 | |||
ORP-1 Antibody (B-3) Alexa Fluor® 790 | sc-376602 AF790 | 200 µg/ml | $357.00 |