NUBP2 Antibody (A-11): sc-376785

NUBP2 Antibody (A-11) is a mouse monoclonal IgG1 antibody that detects NUBP2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NUBP2, also known as cytosolic Fe/S cluster assembly factor NUBP2, is a crucial 271 amino acid protein that plays a significant role in the assembly of iron-sulfur (Fe/S) clusters, which are vital for the function of various enzymes and proteins within the cell. NUBP2 is predominantly located in both the nucleus and cytoplasm, with notable presence at centrosomes during mitosis, highlighting NUBP2′s importance in cellular division and function. NUBP2 is essential for the maturation of extramitochondrial Fe/S proteins, facilitating the transfer of a labile 4Fe-4S cluster to various apoproteins, thereby influencing numerous biological processes, including cellular respiration and metabolism. The NUBP2 gene is part of the NUBP/MRP gene subfamily of ATP-binding proteins and is mapped to human chromosome 16p13.3 and mouse chromosome 17 A3.3, indicating evolutionary conservation and significance across species. With a wide expression profile, particularly in skeletal muscle, fetal liver, lung, brain, and kidney, anti-NUBP2 antibody (A-11) serves as a valuable tool for researchers investigating the intricate roles of Fe/S proteins in health and disease.

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NUBP2 Antibody (A-11) References:

1. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes.  |  Nakashima, H., et al. 1999. Genomics. 60: 152-60. PMID: 10486206
2. Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.  |  Christodoulou, A., et al. 2006. J Cell Sci. 119: 2035-47. PMID: 16638812
3. Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis.  |  Stehling, O., et al. 2008. Mol Cell Biol. 28: 5517-28. PMID: 18573874
4. A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.  |  Ververis, A., et al. 2016. Cell Mol Life Sci. 73: 163-84. PMID: 26153462
5. Genomewide meta-analysis identifies loci associated with IGF-I and IGFBP-3 levels with impact on age-related traits.  |  Teumer, A., et al. 2016. Aging Cell. 15: 811-24. PMID: 27329260
6. A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.  |  Johnson, NB., et al. 2017. J Biol Chem. 292: 15976-15989. PMID: 28768766
7. Function and crystal structure of the dimeric P-loop ATPase CFD1 coordinating an exposed [4Fe-4S] cluster for transfer to apoproteins.  |  Stehling, O., et al. 2018. Proc Natl Acad Sci U S A. 115: E9085-E9094. PMID: 30201724
8. High Content Imaging of Barrett's-Associated High-Grade Dysplasia Cells After siRNA Library Screening Reveals Acid-Responsive Regulators of Cellular Transitions.  |  Phipps, SM., et al. 2020. Cell Mol Gastroenterol Hepatol. 10: 601-622. PMID: 32416156
9. An Anticancer Rhenium Tricarbonyl Targets Fe-S Cluster Biogenesis in Ovarian Cancer Cells.  |  Neuditschko, B., et al. 2022. Angew Chem Int Ed Engl. 61: e202209136. PMID: 36004624
10. Metformin inhibits oral squamous cell carcinoma progression through regulating RNA alternative splicing.  |  Ji, M., et al. 2023. Life Sci. 315: 121274. PMID: 36509195

The preferred NUBP2 antibody is NUBP2 Antibody (C-12): sc-376784.