NRIP3 CRISPR/Cas9 KO Plasmid (h): sc-412715
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NRIP3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NRIP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NRIP3 CRISPR/Cas9 KO Plasmid (h) | sc-412715 | 20 µg | $397.00 |
Overview
NRIP3 (nuclear receptor interacting protein 3) encodes a nuclear protein reported to interact with nuclear receptor–associated transcriptional machinery, suggesting a role in modulating transcriptional programs that govern cell-state decisions. Through these interactions, NRIP3 is relevant to signaling networks linked to hormone-responsive gene regulation, chromatin-associated processes, and context-dependent control of proliferation and differentiation. Altered regulation of nuclear receptor pathways and transcriptional co-regulators is frequently implicated in oncogenic transcriptional rewiring and other complex disease phenotypes, making NRIP3 a useful target for mechanistic studies of gene regulation. Functional interrogation of NRIP3 can help define how nuclear receptor–coupled transcription integrates with broader cellular signaling to shape downstream gene expression.
NRIP3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NRIP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NRIP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NRIP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NRIP3 protein expression.
This CRISPR knockout system enables efficient generation of NRIP3-deficient cell models for investigation of NRIP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NRIP3 exon(s) critical for NRIP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NRIP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NRIP3 CRISPR/Cas9 KO Plasmid (h) and NRIP3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NRIP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NRIP3 HDR Plasmid (h) and NRIP3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NRIP3 homology arms to support homology-directed repair at defined NRIP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.