Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NQO2 Double Nickase Plasmid (h): sc-402200-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NQO2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NQO2 Double Nickase Plasmid (h) and NQO2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NQO2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NQO2 Antibody (A-5): sc-271665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NQO2 Double Nickase Plasmid (h) | sc-402200-NIC | 20 µg | $410.00 | |||
NQO2 Double Nickase Plasmid (h2) | sc-402200-NIC-2 | 20 µg | $410.00 |
NQO2 (NRH:quinone oxidoreductase 2) encodes a cytosolic flavoprotein that catalyzes two-electron reduction of quinones and related electrophiles, contributing to cellular redox homeostasis and xenobiotic metabolism. By modulating quinone/ROS balance, NQO2 intersects with oxidative stress responses, mitochondrial function, and detoxification pathways that influence DNA damage signaling and cell survival. Altered NQO2 activity has been studied in the context of carcinogenesis, neurodegeneration, and inflammatory phenotypes where redox imbalance and electrophile burden are prominent. Human NQO2 is also a reported interaction node for small molecules and endogenous metabolites, making it a useful target for mechanistic studies of redox-linked signaling.
NQO2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NQO2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NQO2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NQO2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NQO2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.