Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NQO1 Double Nickase Plasmid (h): sc-400326-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NQO1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NQO1 Double Nickase Plasmid (h) and NQO1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NQO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NQO1 Antibody (A180): sc-32793
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NQO1 Double Nickase Plasmid (h) | sc-400326-NIC | 20 µg | $410.00 | |||
NQO1 Double Nickase Plasmid (h2) | sc-400326-NIC-2 | 20 µg | $410.00 |
NQO1 (NAD(P)H quinone dehydrogenase 1) is a cytosolic flavoprotein that catalyzes two-electron reduction of quinones to hydroquinones, limiting redox cycling and reactive oxygen species generation. It functions as a key effector of the NRF2/KEAP1 oxidative stress response and contributes to cellular detoxification, maintenance of redox homeostasis, and protection of macromolecules from electrophilic damage. Through its role in xenobiotic metabolism and antioxidant defenses, NQO1 influences cellular susceptibility to oxidative injury and inflammatory stress. Altered NQO1 activity or expression has been associated with variation in stress tolerance and disease-relevant phenotypes in cancer biology, neurodegeneration, and cardiometabolic dysfunction research.
NQO1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NQO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NQO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NQO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NQO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.