NPY5-R CRISPR/Cas9 KO Plasmid (m): sc-421954
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPY5-R CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPY5-R genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPY5-R CRISPR/Cas9 KO Plasmid (m) | sc-421954 | 20 µg | $397.00 |
Overview
Npy5r encodes neuropeptide Y receptor Y5 (NPY5-R), a Gi/o-coupled GPCR that binds neuropeptide Y and related peptides to modulate intracellular cAMP levels and downstream kinase signaling. In the central nervous system, NPY5-R contributes to regulation of feeding behavior, energy homeostasis, stress responsivity, and neuroendocrine outputs through integration of hypothalamic and limbic circuits. Receptor activation influences synaptic transmission, neuronal excitability, and neuropeptidergic network plasticity, linking Npy5r to pathways controlling appetite and metabolic regulation. Dysregulated NPY signaling has been associated with obesity-related phenotypes and affective-stress processes, making Npy5r a useful target for mechanistic studies in mouse models.
NPY5-R CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Npy5r gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Npy5r together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Npy5r open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPY5-R protein expression.
This CRISPR knockout system enables efficient generation of Npy5r-deficient cell models for investigation of NPY5-R signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Npy5r exon(s) critical for NPY5-R function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Npy5r genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPY5-R CRISPR/Cas9 KO Plasmid (m) and NPY5-R CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Npy5r locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPY5-R HDR Plasmid (m) and NPY5-R HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Npy5r homology arms to support homology-directed repair at defined Npy5r target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.