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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for fast excitatory neurotransmission by glutamate, whereas the NMDA receptors exhibit slow kinesis of Ca2+ ions and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: ε 1, 2, 3, 4 and one ζ subunit. The ζ subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using NMDAε1 (5): sc-136004 antibody for immunoprecipitation, how much antibody should I use?
Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
Rated 5 out of
Strong band in WB in mouse NMDA 1 transfectedStrong band in WB in mouse NMDA 1 transfected 293 whole cell lysate and mouse brain and mouse cerebellum tissue extracts. -SCBT QC
Date published: 2015-02-02
Rated 5 out of
Good band in WB in mouse cerebrum tissue extractGood band in WB in mouse cerebrum tissue extract. -SCBT QC
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