NMDA epsilon 1 Antibody (5) recommended for detection of NMDAε1 of mouse and rat origin by WB and IP

NMDAε1 Antibody (5): sc-136004

NMDAε1 Antibody (5) is rated 5.0 out of 5 by 2.
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Datasheets

    • NMDAε1 Antibody (5) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 1093-1214 of NMDAε1 of mouse origin
    • recommended for detection of NMDAε1 of mouse and rat origin by WB and IP

I am using NMDAε1 (5): sc-136004 antibody for immunoprecipitation, how much antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Strong band in WB in mouse NMDA 1 transfected Strong band in WB in mouse NMDA 1 transfected 293 whole cell lysate and mouse brain and mouse cerebellum tissue extracts. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Good band in WB in mouse cerebrum tissue extract Good band in WB in mouse cerebrum tissue extract. -SCBT QC
Date published: 2014-12-03
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