NLE1 Antibody (G-5): sc-377142
- NLE1 Antibody (G-5) is a mouse monoclonal IgG1 κ NLE1 antibody provided at 200 µg/ml
- raised against amino acids 1-240 mapping at the N-terminus of NLE1 of human origin
- NLE1 Antibody (G-5) is recommended for detection of NLE1 of human origin by WB, IP, IF and ELISA
- Anti-NLE1 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-377142 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for NLE1 Antibody (G-5) for WB applications. This reagent is now offered in a bundle with NLE1 Antibody (G-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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NLE1 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NLE1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NLE1 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NLE1, also known as notchless homolog 1, is a 485 amino acid WD40-repeat protein that plays a critical role in regulating the Notch signaling pathway, which is essential for proper cell communication during development. NLE1 is primarily located in the cytoplasm, where NLE1 interacts with the cytoplasmic domain of Notch receptors, thereby modulating their signaling activity. NLE1′s cellular location is important for influencing cell fate decisions and developmental processes; deletion of NLE1 during early embryonic development in mice leads to embryonic lethality, while later deletion activates a caspase-3-dependent apoptotic pathway, highlighting NLE1′s vital role in maintaining cellular integrity. In plants, NLE1 is crucial for normal growth and development, with under-expression during shoot proliferation resulting in delayed flowering and abnormal organ maturation. Additionally, NLE1 may be involved in the biogenesis of the 60S ribosomal subunit in yeast. NLE1′s structure, characterized by eight WD40 domains, allows for versatile interactions, and NLE1 produces one isoform through alternative splicing, further emphasizing NLE1′s functional significance in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NLE1 Antibody (G-5) References:
- Wdr12, a mouse gene encoding a novel WD-Repeat Protein with a notchless-like amino-terminal domain. | Nal, B., et al. 2002. Genomics. 79: 77-86. PMID: 11827460
- The murine ortholog of notchless, a direct regulator of the notch pathway in Drosophila melanogaster, is essential for survival of inner cell mass cells. | Cormier, S., et al. 2006. Mol Cell Biol. 26: 3541-9. PMID: 16611995
- Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development. | Chantha, SC., et al. 2006. Plant Mol Biol. 62: 897-912. PMID: 17006595
- Underexpression of the plant NOTCHLESS gene, encoding a WD-repeat protein, causes pleitropic phenotype during plant development. | Chantha, SC. and Matton, DP. 2007. Planta. 225: 1107-20. PMID: 17086402
- Notchless encodes a novel WD40-repeat-containing protein that modulates Notch signaling activity. | Royet, J., et al. 1998. EMBO J. 17: 7351-60. PMID: 9857191
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NLE1 Antibody (G-5) | sc-377142 | 200 µg/ml | $322.00 | |||
NLE1 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-523209 | 200 µg Ab, 40 µg BP | $361.00 | |||
NLE1 Antibody (G-5) X | sc-377142 X | 200 µg/0.1 ml | $322.00 | |||
NLE1 Antibody (G-5) AC | sc-377142 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NLE1 Antibody (G-5) HRP | sc-377142 HRP | 200 µg/ml | $322.00 | |||
NLE1 Antibody (G-5) FITC | sc-377142 FITC | 200 µg/ml | $336.00 | |||
NLE1 Antibody (G-5) PE | sc-377142 PE | 200 µg/ml | $349.00 | |||
NLE1 Antibody (G-5) Alexa Fluor® 488 | sc-377142 AF488 | 200 µg/ml | $364.00 | |||
NLE1 Antibody (G-5) Alexa Fluor® 546 | sc-377142 AF546 | 200 µg/ml | $364.00 | |||
NLE1 Antibody (G-5) Alexa Fluor® 594 | sc-377142 AF594 | 200 µg/ml | $364.00 | |||
NLE1 Antibody (G-5) Alexa Fluor® 647 | sc-377142 AF647 | 200 µg/ml | $364.00 | |||
NLE1 Antibody (G-5) Alexa Fluor® 680 | sc-377142 AF680 | 200 µg/ml | $364.00 | |||
NLE1 Antibody (G-5) Alexa Fluor® 790 | sc-377142 AF790 | 200 µg/ml | $364.00 |