NFKBIA/IkB alpha Double Nickase Plasmid (m): sc-421878-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFKBIA/IkB alpha Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFKBIA/IkB alpha Double Nickase Plasmid (m) and NFKBIA/IkB alpha Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nfkbia. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFKBIA/IkB alpha Antibody (H-4): sc-1643
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFKBIA/IkB alpha Double Nickase Plasmid (m) | sc-421878-NIC | 20 µg | $410.00 |
Mouse Nfkbia encodes NFKBIA/IκBα, a cytoplasmic inhibitor that binds NF-κB dimers (including RELA/p50) and retains them outside the nucleus under basal conditions. Upon inflammatory or stress signaling, IKK-dependent phosphorylation triggers ubiquitination and proteasomal degradation of IκBα, permitting NF-κB nuclear translocation and transcriptional control of cytokines, survival factors, and feedback regulators. This protein is central to canonical NF-κB signaling and intersects with TNF, IL-1, TLR, and antigen receptor pathways, shaping innate and adaptive immune responses. Dysregulated Nfkbia function or altered IκBα turnover has been linked to aberrant inflammation, immune cell activation states, and oncogenic signaling contexts where NF-κB activity is constitutively engaged.
NFKBIA/IkB alpha Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nfkbia locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nfkbia. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nfkbia function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nfkbia-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.